The mass production and use of organophosphorus pesticides (OPs) have led to a threat to human health. Therefore, establishing a sensitive, rapid, and high-throughput detection method is of great importance. In this study, computer-aided molecular design was firstly applied to design the specific haptens of phorate (PHO), fenthion (FEN), and profenofos (PRO), and high-performance monoclonal antibodies against PHO, FEN, and PRO were prepared. On this basis, a multiplex fluorescent microsphere immunochromatographic assay (FM-ICA) was developed for simultaneous detection of three OPs in cowpea and orange samples. The quantitative detection ranges of PHO, FEN, and PRO were 9.039-140.129, 6.686-108.613, and 5.215-71.479 μg/kg, respectively, in cowpeas; and 7.120-121.566, 3.592-92.185, and 3.579-60.081 μg/kg, respectively, in oranges. The results of recovery and real sample testing showed that the multiplex FM-ICA was rapid and accurate, making it suitable for the on-site testing of a large number of samples.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.foodchem.2025.142850 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A multiplex fluorescent microsphere immunochromatography assay for simultaneous detection of phorate, fenthion, and profenofos.
Food Chem
January 2025
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. Electronic address:
The mass production and use of organophosphorus pesticides (OPs) have led to a threat to human health. Therefore, establishing a sensitive, rapid, and high-throughput detection method is of great importance. In this study, computer-aided molecular design was firstly applied to design the specific haptens of phorate (PHO), fenthion (FEN), and profenofos (PRO), and high-performance monoclonal antibodies against PHO, FEN, and PRO were prepared.
View Article and Find Full Text PDFElectropositive Magnetic Fluorescent Nanoprobe-Mediated Immunochromatographic Assay for the Ultrasensitive and Simultaneous Detection of Bacteria.
Adv Sci (Weinh)
January 2025
Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510000, China.
Immunochromatographic assays (ICAs) provide simple and rapid strategies for bacterial diagnosis but still suffer from the problems of low sensitivity and high dependency on paired antibodies. Herein, the broad-spectrum capture and detection capability of the antibody-free electropositive nanoprobe are clarified for bacteria for the first time and an ultrasensitive fluorescent ICA platform is constructed for the simultaneous diagnosis of multiple pathogens. A magnetic multilayer quantum dot nanocomposite with an amino-embedded SiO shell (MagMQD@Si) is designed to enrich bacteria from solutions effectively, offer high luminescence, and reduce background signals on test strips, thus greatly improving the sensitivity and stability of ICA technique for pathogen.
View Article and Find Full Text PDFThe discovery of broadly protective antibodies to the influenza virus neuraminidase (NA) has raised interest in NA as a vaccine target. However, recombinant, solubilized tetrameric NA ectodomains are often challenging to express and isolate, hindering the study of anti-NA humoral responses. To address this obstacle, we established a panel of 22 non-adherent cell lines stably expressing native, historical N1, N2, N3, N9, and NB NAs anchored on the cell surface.
View Article and Find Full Text PDFDeveloping Orthogonal Fluorescent RNAs for Photoactive Dual-color Imaging of RNAs in Live Cells.
Angew Chem Int Ed Engl
January 2025
Hunan University, College of Chemistry and Chemical Engineering, Yuelushan, Changsha, Hunan, 410082, P.R.China, 410082, Changsha, CHINA.
Fluorogenic RNA aptamers have revolutionized the visualization of RNAs within complex cellular processes. A representative category of them employs the derivatives of green fluorescent protein chromophore, 4-hydroxybenzlidene imidazolinone (HBI), as chromophores. However, the structural homogeneity of their chromophoric backbones causes severe cross-reactivity with other homologous chromophores.
View Article and Find Full Text PDFDeterministic patterns in single-cell transcriptomic data.
NPJ Syst Biol Appl
January 2025
School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
We report the existence of deterministic patterns in statistical plots of single-cell transcriptomic data. We develop a theory showing that the patterns are neither artifacts introduced by the measurement process nor due to underlying biological mechanisms. Rather they naturally emerge from finite sample size effects.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!