Spatiotemporal generation of alternating disparate pH domains audible sound controlled opposing enzymatic reactions.

Intracellular spatiotemporal chemical heterogeneities with controlled properties are essential for life. However, creating these heterogeneities artificially is challenging. In this study, we used both acid- and base-producing enzymatic reactions simultaneously and demonstrated that the execution of these reactions in the presence of audible sound can effectively create spatiotemporally ordered pH domains in a solution.