Synthetic biology meets Aspergillus: engineering strategies for next-generation organic acid production.

World J Microbiol Biotechnol

School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, No. 1 Wenyuan Road, Nanjing, 210023, People's Republic of China.

Published: January 2025

Organic acids constitute a vital category of chemical raw materials. They have extensive applications in industries such as polymers, food, and pharmaceuticals. Currently, industrial production predominantly relies on microbial fermentation. Aspergillus, due to its unique metabolic capabilities, has become an important microbial resource for organic acid production. In recent years, there has been a growing emphasis on genetic engineering of Aspergillus to increase its yield of organic acids. This review provides a comprehensive overview of the current advancement and future directions in the application of genetic engineering techniques to enhance organic production in Aspergillus, specifically highlighting achievement in reconstructing metabolic pathways for desired products, eliminating by-products, modifying regulatory pathways, and engineering mycelial morphology. Furthermore, this review also focuses on the strategies and genetic tools applied in Aspergillus, with particular emphasis on the potential applications and challenges of CRISPR-based biosensors in organic acid fermentation. By providing insights into these developments, we aim to offer theoretical guidance and innovative approaches for enhancing the efficiency of Aspergillus strains in industrial organic acid production.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11274-024-04246-xDOI Listing

Publication Analysis

Top Keywords

organic acid
16
acid production
12
organic acids
8
genetic engineering
8
organic
7
aspergillus
6
production
5
synthetic biology
4
biology meets
4
meets aspergillus
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!