The alcoholic fermentation of wine is mostly achieved by the species Saccharomyces cerevisiae that display a large variability for their ability to consume or produce malic acid. To better characterize the metabolism of such group of strains we explored their non-volatile metabolome using an untargeted LC-HRMS approach. The chemical classes and the putative structures of several hundred compounds where annotated using MS2 spectra using the SIRIUS software. By using both supervised and univariate statistical analyses, several metabolic features able to discriminate the two group of strains in several wines were listed. Quantitative enrichment analyses pointed out drastic differences in pantothenic acid metabolism between the two group of strains. In addition, the produced showed important change in their nitrogen composition that might be due to the pH difference in the resulting wines. Altogether this work paves the avenue for better characterizing the biochemical impact of yeast strains that modulate wine acidity.
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http://dx.doi.org/10.1016/j.foodchem.2024.142686 | DOI Listing |
J Am Chem Soc
January 2025
Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, Ed. I+D+i. Mariano Esquillor, Zaragoza 50018, Spain.
DNA nanotechnology is a powerful synthetic approach to crafting diverse nanostructures through self-assembly. Chemical decoration of such nanostructures is often required to tailor their properties for specific applications. In this Letter, we introduce a pioneering method to direct the assembly and enable the functionalization of DNA nanostructures using an azide-bearing functional polyamine.
View Article and Find Full Text PDFVet Res Commun
January 2025
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Post Box No. 6450, Yelahanka, Bengaluru, Karnataka, 560119, India.
Sheeppox and Goatpox are highly contagious transboundary viral diseases of sheep and goats caused by Capripoxvirus, respectively. This study describes the development of indirect ELISA and its serodiagnostic potential as an alternative to the gold standard serum neutralization test (SNT). The homologue of vaccinia virus, ORF 117 (A27L) gene of the Romanian Fenner (RF) strain of Sheeppox virus (SPPV) was used for producing the full-length recombinant A27L (rA27L) protein (∼22 kDa) in a prokaryotic expression system.
View Article and Find Full Text PDFAntonie Van Leeuwenhoek
January 2025
Department of Marine Science and Technology, Fukui Prefectural University, Obama, Fukui, 917-0003, Japan.
A novel aerobic marine bacterium, FRT2, isolated from surface water of a fishing port in Fukui, Japan, was characterised based on phylogenomic and phylogenetic analyses combined with classical phenotypic and chemotaxonomic characterisations. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FRT2 clustered with genus Leeuwenhoekiella. Closest relatives of FRT2 were Leeuwenhoekiella palythoae KMM 6264 and Leeuwenhoekiella nanhaiensis G18 with 16S rRNA gene sequence identities of 95.
View Article and Find Full Text PDFPhysiol Genomics
January 2025
Research Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany.
Decades of artificial selection have markedly enhanced egg production efficiency, yet the epigenetic underpinnings, notably DNA methylation dynamics in the gut, remain largely unexplored. Here, we investigate how breeds and developmental stages influence DNA methylation profiles in laying hens, and their potential relationship to laying performance and gut health. We compared two highly selected laying hen strains, Lohmann Brown-Classic (LB) and Lohmann LSL-Classic (LSL), which exhibited similar egg production but divergent physiological, metabolic, and immunological characteristics.
View Article and Find Full Text PDFHum Vaccin Immunother
December 2025
Crucell Integration, Janssen Research and Development, Beerse, Belgium.
We conducted a randomized, Phase 2 trial to assess the safety and humoral immunogenicity of reduced doses/dose volume of the standard dose of Ad26.COV2.S COVID-19 vaccine (5 × 10 viral particles [vp]) in healthy adolescents aged 12-17 years.
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