AI Article Synopsis

  • Pectin is a complex substance in plant cell walls, crucial for breaking down in animal feed to enhance nutrient absorption.
  • Significant amounts of pectin are found in soybean meal, a common poultry feed, but its structure and the necessary enzymes for degradation are not well understood.
  • The study developed and tested various combinations of fungal enzymes, identifying 10 effective ones for breaking down soybean meal pectin, mainly from the fungus Talaromyces versatilis, and proposes a new structural model for understanding pectin in feed.

Article Abstract

Pectin is a complex plant heteropolysaccharide whose structure and function differ depending on its source. In animal feed, breaking down pectin is essential, as its presence increases feed viscosity and reduces nutrient absorption. Soybean meal, a protein-rich poultry feed ingredient, contains significant amounts of pectin, the structure of which remains unclear. Consequently, the enzyme activities required to degrade soybean meal pectin and how they interact are still open questions. In this study, we produced 15 recombinant fungal carbohydrate-active enzymes (CAZymes) identified from fungal secretomes acting on pectin. After observing that these enzymes were not active on soybean meal pectin when used alone, we developed a semi-miniaturized method to evaluate their effect as multi-activity cocktails. We designed and tested 12 enzyme pools, containing up to 15 different CAZymes, using several hydrolysis markers. Thanks to our multiactivity enzymatic approach combined with a Pearson correlation matrix, we identified 10 fungal CAZymes efficient on soybean meal pectin, 9 of which originate from Talaromyces versatilis. Based on enzyme specificity and linkage analysis, we propose a structural model for soybean meal pectin. Our findings underscore the importance of combining CAZymes to improve the degradation of agricultural co-products.

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http://dx.doi.org/10.1038/s41598-024-83289-4DOI Listing

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Article Synopsis
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  • The study developed and tested various combinations of fungal enzymes, identifying 10 effective ones for breaking down soybean meal pectin, mainly from the fungus Talaromyces versatilis, and proposes a new structural model for understanding pectin in feed.
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