AI Article Synopsis
- The study explored how human vocal fold fibroblasts (hVFF) interact with macrophages in the presence of cigarette smoke extract (CSE) and vibration, focusing on vocal fold inflammation.
- Researchers cultured hVFF with CSE, applying either static or dynamic conditions, and then measured various mRNA and protein levels to assess inflammation.
- Findings revealed that vibration may reduce CSE-induced inflammatory responses in hVFF, suggesting potential mechanisms to address voice disorders linked to smoking-related inflammation.
Article Abstract
Objectives: This in vitro study investigated the interaction between human vocal fold fibroblasts (hVFF) and macrophages under the influence of cigarette smoke extract (CSE) and vibration as potential regulators of vocal fold (VF) inflammation.
Study Design: Experimental in vitro pilot study.
Methods: Immortalized hVFF were cultured in flexible-bottomed cell culture plates, treated with CSE, and subjected to static or dynamic conditions in a phonomimetic bioreactor. For coculture, unstimulated or lipopolysaccharide/IFNγ-stimulated THP-1 (human leukemia monocytic cell line) macrophages were added in inserts for a final 24 hours of vibration period. We measured messenger ribonucleic acid (mRNA) (quantitative polymerase chain reaction [qPCR]) and protein levels (Western Blot, ELISA, and LUMINEX®) of hVFF and analyzed the results using two- and three-way ANOVA with post hoc tests.
Results: Under inflammatory stimulation, we observed a reduction of collagen (COL) type 1A1, 1A2, and 3A1, and increased gene expression of COL4A1, matrix metallopeptidase 2, and vascular endothelial growth factor A in hVFF. Additionally, the pro-inflammatory markers cyclooxygenase (COX) 1 and 2, interleukin (IL) 1β, IL-6, and IL-8 were upregulated. CSE increased COX1 and COX2 levels, whereas vibration reduced CSE-induced increases of COL4A1 and COX2 in pro-inflammatory stimulated hVFF.
Conclusion: This study indicates that vibration may mitigate CSE-induced inflammatory damage in the hVFF, thereby offering new insights into the cellular crosstalk that underlies the pathophysiology of VF inflammation in smoking-related voice disorders.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jvoice.2024.12.043 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Functional Characteristics of the Crosstalk Between Vocal Fold Fibroblasts and Macrophages-The Role of Vibration in Vocal Fold Inflammation.
J Voice
January 2025
Division of Phoniatrics, ENT University Hospital Graz, Medical University of Graz, Graz, Austria; Division of Physiology and Pathophysiology, Otto Loewi Research Center for Vascular Biology, Immunology and Inflammation, Medical University of Graz, Graz, Austria.
Article Synopsis
- The study explored how human vocal fold fibroblasts (hVFF) interact with macrophages in the presence of cigarette smoke extract (CSE) and vibration, focusing on vocal fold inflammation.
- Researchers cultured hVFF with CSE, applying either static or dynamic conditions, and then measured various mRNA and protein levels to assess inflammation.
- Findings revealed that vibration may reduce CSE-induced inflammatory responses in hVFF, suggesting potential mechanisms to address voice disorders linked to smoking-related inflammation.
DaxibotulinumtoxinA Treatment for Laryngeal Respiratory Dystonia.
J Voice
January 2025
Department of Otolaryngology-Head and Neck Surgery, UCSF Voice and Swallowing Center, UCSF School of Medicine, San Francisco, CA. Electronic address:
Background: Laryngeal respiratory dystonia (LRD) is diagnosed based on clinical presentation, patient history, and physical examination. Key indicators include dyspnea, desynchronized breathing patterns, and laryngoscopic findings that reveal vocal fold adduction during inspiration. Treatment for LRD remains controversial and often yields limited effectiveness.
View Article and Find Full Text PDFAnalysis and Categorization of Various Types of Vocal Distortion in Rock, Metal, Pop Styles, and Throat Singing Observed by High-Speed Digital Imaging.
J Voice
January 2025
Faculty of Design, Kyushu University, 4-9-1 Shiobaru, Minami-ku, Fukuoka 815-8540, Japan.
Introduction: Vocal distortion, also known as a scream or growl, is used worldwide as an essential technique in singing, especially in rock and metal, and as an ethnic voice in Mongolian singing. However, the production mechanism of vocal distortion is not yet clearly understood owing to limited research on the behavior of the larynx, which is the source of the distorted voice.
Objectives: This study used high-speed digital imaging (HSDI) to observe the larynx of professional singers with exceptional singing skills and determine the laryngeal dynamics in the voice production of various vocal distortions.
The impact of music on patient tolerance during office-based laryngeal surgery.
Laryngoscope Investig Otolaryngol
February 2025
Objective: To investigate the impact of music on patient tolerance during office-based laryngeal surgery (OBLS).
Methods: All patients undergoing OBLS between February 2024 to June 2024 were invited to participate in this study. They were divided into two subgroups, those with music in the background during surgery and those without.
Elastic cartilage properties of the tip of the vocal process of the arytenoid cartilage.
Laryngoscope Investig Otolaryngol
February 2025
Objectives: This study aimed to investigate the histological and ultrastructural features of the elastic cartilage at the tip of the vocal process in the arytenoid cartilage, which is essential for laryngeal biomechanics.
Methods: Five larynges, including the vocal folds and epiglottis, were examined using transmission electron microscopy. The elastic cartilage at the tip of the vocal process was compared to the epiglottic cartilage within the same larynx to elucidate structural differences.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!