Paeniclostridium sordellii is responsible for severe infections in horses, cattle, and sheep; however, conventional vaccines exhibit limitations in production and immunogenicity. This study assessed the immunogenicity of a recombinant bacterin composed of a chimera (rQTcsHL) that combines segments from the lethal (TcsL) and hemorrhagic (TcsH) toxins in mice and sheep. Both immunized animal groups exhibited elevated levels of IgG, with the mice demonstrating moderate protection (<50%) against lethal challenges, comparable to that of the conventional vaccine. Further molecular optimization is essential to enhance its efficacy.
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College of Mathematical Sciences, College of Science, Rochester Institute of Technology, Rochester, NY.
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Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Brasil.
Paeniclostridium sordellii is responsible for severe infections in horses, cattle, and sheep; however, conventional vaccines exhibit limitations in production and immunogenicity. This study assessed the immunogenicity of a recombinant bacterin composed of a chimera (rQTcsHL) that combines segments from the lethal (TcsL) and hemorrhagic (TcsH) toxins in mice and sheep. Both immunized animal groups exhibited elevated levels of IgG, with the mice demonstrating moderate protection (<50%) against lethal challenges, comparable to that of the conventional vaccine.
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