In this study, we fully sequenced and analyzed the genome of strain 12219 and identified it as Streptomyces thermocarboxydus. The genome contained a single linear chromosome, 6,950,031 bp in size, with a GC content of 72.21 %. This study predicted a total of 6295 genes, including 128 glycoside hydrolase genes, 21 carbohydrate esterase genes, and 54 carbohydrate-binding module genes. When corncob was used as inducer, strain 12219 secreted cellulases and hemicellulases, with xylanase activity reaching 31.15 U/mL. During the hydrolysis of sodium hydroxide treated corn stover, a notable synergistic effect between the 12219 enzyme cocktail and commercial cellulase was observed. And the maximum degree of synergism reached 1.60. When the amount of the 12219 enzyme cocktail added to the commercial cellulase was 5 mg/g, the release of glucose, xylose, and cellobiose increased by 121.35 %, 178.58 %, and 29.33 %, respectively. These findings suggested that the 12219 enzyme cocktail held great potential for industrial applications.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2025.139675 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genomic analysis and synergistic effect with cellulase by Streptomyces thermocarboxydus 12219.
Int J Biol Macromol
January 2025
College of Forestry, Henan Agricultural University, Zhengzhou 450046, China. Electronic address:
In this study, we fully sequenced and analyzed the genome of strain 12219 and identified it as Streptomyces thermocarboxydus. The genome contained a single linear chromosome, 6,950,031 bp in size, with a GC content of 72.21 %.
View Article and Find Full Text PDFEnhancing Phycocyanobilin Production Efficiency in Engineered : Strategies and Potential Application.
J Agric Food Chem
May 2024
Department of Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Phycocyanobilin, an algae-originated light-harvesting pigment known for its antioxidant properties, has gained attention as it plays important roles in the food and medication industries and has surged in demand owing to its low-yield extraction from natural resources. In this study, engineered was developed to achieve high PCB production, and three strategies were proposed: reinforcement of the heme biosynthesis pathway with the introduction of two PCB-related enzymes, strengthening of the pentose phosphate pathway to generate an efficient cycle of NADPH, and fed-batch fermentation to maximize PCB production. Each approach increased PCB synthesis, and the final engineered strain successfully produced 78.
View Article and Find Full Text PDFBiosensors and bioassays, both of which employ proteins and nucleic acids to detect specific molecular targets, have seen significant applications in both biomedical research and clinical practice. This success is largely due to the extraordinary versatility, affinity, and specificity of biomolecular recognition. Nevertheless, these receptors suffer from an inherent limitation: single, saturable binding sites exhibit a hyperbolic relationship (the "Langmuir isotherm") between target concentration and receptor occupancy, which in turn limits the sensitivity of these technologies to small variations in target concentration.
View Article and Find Full Text PDFDesign, Synthesis, and Biological Evaluation of Dual-Target COX-2/CYP51 Inhibitors for the Treatment of Fungal Infectious Diseases.
J Med Chem
September 2022
Institute of BioPharmaceutical Research, Liaocheng University, 1 Hunan Road, Liaocheng 252000, P. R. China.
The design of novel dual-target (COX-2/CYP51) inhibitors was proposed in the study, and three series of compounds were constructed though the pathway of skeleton screening and combination; their molecular structures were synthesized and evaluated. Most of the compounds exhibited significant antifungal ability. Among them, potential compounds (, ) with excellent antifungal and anti-drug-resistant fungal ability (MIC, 0.
View Article and Find Full Text PDFNanostructure-based functions are omnipresent in nature and essential for the diversity of life. Unlike small molecules, which are often inhibitors of enzymes or biomimetics with established methods of elucidation, we show that functions of nanoscale structures in cells are complex and can implicate system-level effects such as the regulation of energy and redox homeostasis. Herein, we design a platinum(II)-containing tripeptide that assembles into intracellular fibrillar nanostructures upon molecular rearrangement in the presence of endogenous HO.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!