The human skin commensal produces diverse, therapeutically relevant bacteriocins. We report the complete whole-genome sequence of the nasal isolate B273, which contains a plasmid with the biosynthetic gene cluster for epidermicin NI01, a broad-spectrum type II antimicrobial peptide.
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Complete genome sequence of B273 and its epidermicin NI01 biosynthesis plasmid.
Microbiol Resour Announc
January 2025
Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.
The human skin commensal produces diverse, therapeutically relevant bacteriocins. We report the complete whole-genome sequence of the nasal isolate B273, which contains a plasmid with the biosynthetic gene cluster for epidermicin NI01, a broad-spectrum type II antimicrobial peptide.
View Article and Find Full Text PDFThe fluorescent protein iLOV as a reporter for screening of high-yield production of antimicrobial peptides in Pichia pastoris.
Microb Biotechnol
July 2022
Ingenza Ltd, Roslin Innovation Centre, Charnock Bradley Building, Roslin, EH25 9RG, UK.
The methylotrophic yeast Pichia pastoris is commonly used for the production of recombinant proteins at scale. The identification of an optimally overexpressing strain following transformation can be time and reagent consuming. Fluorescent reporters like GFP have been used to assist identification of superior producers, but their relatively big size, maturation requirements and narrow temperature range restrict their applications.
View Article and Find Full Text PDFFlowering Poration-A Synergistic Multi-Mode Antibacterial Mechanism by a Bacteriocin Fold.
iScience
August 2020
National Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK; Department of Physics, King's College London, Strand Lane, London WC2R, UK. Electronic address:
Bacteriocins are a distinct family of antimicrobial proteins postulated to porate bacterial membranes. However, direct experimental evidence of pore formation by these proteins is lacking. Here we report a multi-mode poration mechanism induced by four-helix bacteriocins, epidermicin NI01 and aureocin A53.
View Article and Find Full Text PDFA single dose of epidermicin NI01 is sufficient to eradicate MRSA from the nares of cotton rats.
J Antimicrob Chemother
March 2017
Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
Objectives: To investigate the efficacy of a potent novel antimicrobial protein of mass 6 kDa, epidermicin NI01, for eradicating the nasal burden of MRSA in a cotton rat ( Sigmodon hispidus ) model.
Methods: MRSA strain ATCC 43300 was used to establish a robust colonization of cotton rat nares. This model was used to evaluate the efficacy of topical 0.
Antimicrobial Peptides as Anti-Infectives against Staphylococcus epidermidis.
Med Princ Pract
August 2017
Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India.
Staphylococcus epidermidis has emerged as the main causative agent for graft-related and nosocomial infections. Rampant use of antibiotics and biofilm formed by the organism results in poor penetration of the drug and further aggravates the antibiotic resistance, emphasizing an urgent need to explore alternative treatment modalities. Antimicrobial peptides (AMPs), produced as effector molecules of the innate immunity of living organisms, have therapeutic potential that can be used to inhibit the growth of microbes.
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