Six different freezing/thawing programs, which varied freezing rate, duration of freezing, and thawing rates, were used to investigate the effect of these factors on cell destruction in dog skin. The range of tissue temperatures produced was from -15 to -50 degrees C. The extent of destruction was evaluated by skin biopsies 3 days after cold injury. In single, short freezing/thawing cycles, the temperature reached in the tissue was the prime factor in cell death. Longer freezing time and slow thawing were also important lethal factors which increased destruction of cells. Cooling rate, whether slow or fast, made little difference in the outcome. The experiments suggested that present-day, commonly employed cryosurgical techniques, which feature fast cooling, slow thawing, and repetition of the freeze/thaw cycle, should be modified by the use of maintenance of the tissue in the frozen state for several minutes and slow thawing. Thawing should be complete before freezing is repeated. These modifications in technique will maximize tissue destruction, an important consideration in cancer cryosurgery.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0011-2240(85)90172-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative effectiveness of vitrification and slow freezing after heterotopic transplantation of human ovarian tissues.
BMC Womens Health
December 2024
Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, China.
Background: The aim of this study was to compare the effectiveness of two different vitrification methods and slow freezing in terms of the recovery of endocrine function, follicular morphology and proliferation, apoptosis of stromal cells, and angiogenesis after heterotopic transplantation of human ovarian tissue.
Methods: Ovarian tissue from young women aged 29 to 40 was subjected to two vitrification methods and one slow freezing method. The thawed ovarian tissue was then transplanted into nude mice and divided into three groups (VF1 group, VF2 group, SF group) according to the different freezing methods.
Enhanced cell survival in prepubertal testicular tissue cryopreserved with membrane lipids and antioxidants rich cryopreservation medium.
Cell Tissue Res
November 2024
Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.
The present study explores the advantages of enriching the freezing medium with membrane lipids and antioxidants in improving the outcome of prepubertal testicular tissue cryopreservation. For the study, testicular tissue from Swiss albino mice of prepubertal age group (2 weeks) was cryopreserved by slow freezing method either in control freezing medium (CFM; containing DMSO and FBS in DMEM/F12) or test freezing medium (TFM; containing soy lecithin, phosphatidylserine, phosphatidylethanolamine, cholesterol, vitamin C, sodium selenite, DMSO and FBS in DMEM/F12 medium) and stored in liquid nitrogen for at least one week. The tissues were thawed and enzymatically digested to assess viability, DNA damage, and oxidative stress in the testicular cells.
View Article and Find Full Text PDFExploring cryopreservation alternatives for Dirofilaria immitis microfilariae.
Vet Parasitol
January 2025
Department of Veterinary Science, University of Messina, Polo Universitario dell'Annunziata, Messina 98168, Italy.
Canine Heartworm Disease, caused by Dirofilaria immitis, primarily affects canids and felids. The earliest studies on cryopreservation were carried out at -70°C, achieving acceptable survival rates, however microfilariae (mf) showed alterations both in morphology and motility. Thereafter, liquid nitrogen was used representing an excellent tool for long-term preservation, albeit it is expensive and requires trained personnel.
View Article and Find Full Text PDFAnethole improves mitochondrial activity and quality parameters in fresh and frozen-thawed ovine semen.
Res Vet Sci
December 2024
Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Cep 24230-340, Niterói, RJ, Brazil. Electronic address:
Anethole, an antioxidant found in plants, appears to improve the survival of spermatozoa during semen cryopreservation. This study assessed the effects of commercial trans-anethole in ram semen cryopreservation. Thirty ejaculates from six rams were diluted in media containing anethole at the following concentrations: CONT (0 μM), AN10 (10 μM), AN50 (50 μM), and AN100 (100 μM).
View Article and Find Full Text PDFInvestigation of the efficacy of different cryoprotectants in the freezing of testicular tissue and epididymal sperm: Spermatological parameters, tissue viability and PARP-1 gene expression.
Cryobiology
December 2024
University of Ondokuz Mayis, Department of Animal Reproduction and Artificial Insemination, Samsun, 55200, Turkey. Electronic address:
Article Synopsis
- The study focuses on the cryopreservation of testicular tissue and sperm in bulls, examining how these processes impact sperm quality and cell viability, as well as the expression of the PARP-1 gene.
- Testes were collected from 20 bulls, and sperm was frozen using liquid nitrogen, with testicular tissue treated with cryoprotectants like DMSO and EG before freezing.
- Results showed that post-thaw sperm motility and viability significantly decreased, with DMSO improving cell viability more than EG, and both cryoprotectants influenced gene expression related to DNA repair.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!