LGR5 as a diagnostic marker for dysplasia in serrated polyps.

Aims: WNT signalling pathway dysregulation is often a critical early component in colorectal neoplasia, particularly the chromosomal instability pathway. Using two WNT reporters, and , we sought to assess whether these polyps demonstrate predictable expression patterns and if these patterns show diagnostic value.

Methods: We evaluated 23 adenomas (TA), 23 sessile serrated lesions (SSLs), 14 SSL with dysplasia and 38 traditional serrated adenomas (TSA). Chromogenic in situ hybridisation stains (ISH) for and were performed. Reactivity was defined as strong, intermediate or weak. Upper third crypt reactivity was defined as full-thickness staining. Accentuation within ectopic crypts (ECF) was recorded.

Results: TAs (91%) showed strong reactivity and full-thickness staining with . TSAs showed full-thickness and weak to intermediate reactivity (79%) and ECF with accentuation was exclusively seen in TSA. SSL showed weak reactivity confined to the basal crypt region (100%). SSL with dysplasia also showed weak or intermediate (100%) reactivity, but the reactivity pattern was full thickness (88%). expression parallels expression (Pearson coefficient=0.63) regarding signal intensity for the examined polyp groups.

Conclusions: Qualitative and quantitative differences in and expression assist in the diagnosis of SSL with dysplasia.