Green fabricated bimetallic zinc ferrite nanoparticles mitigate oxidative stress-induced pathogenesis.

Current study evaluates the beneficial role of bio-functionalized zinc ferrite nanoparticles fabricated from an aqueous extract of Decalepis hamiltonii leaves (DHLE.ZnFeO NPs) on sodium nitrite (NaNO) and Diclofenac (DFC) induced oxidative stress in RBCs and Sprague Dawley male rat models. DHLE.ZnFeO NPs were characterized using PXRD, FTIR, SEM-EDAX, HR-TEM and VSM. The data suggests that, DHLE.ZnFeO NPs were crystalline, ellipsoidal in shape with an average size of 10.95 nm and super paramagnetic in nature. DHLE.ZnFeO NPs exhibited anti-oxidant properties by scavenging DPPH, HO and reducing ferric to ferrous ions. Furthermore, DHLE.ZnFeO NPs normalized key parameters of oxidative stress such as LPO, PCC, TT and anti-oxidant enzymes (SOD &CAT). Similar to the previous in-vitro results, DHLE.ZnFeO NPs restored all the said stress parameters in homogenates of the liver, kidney, pancreas and heart. In addition, DHLE.ZnFeO NPs repaired Diclofenac induced tissue damage in the liver, kidney, pancreas and heart by regulating all biochemical parameters. Most importantly, DHLE.ZnFeO NPs exhibited anti-inflammatory, anti-diabetic, anti-thrombotic activities and were non-toxic to RBCs. In conclusion, DHLE.ZnFeO NPs through its anti-oxidant potential ameliorate oxidative stress induced pathogenesis such as, inflammation, tissue damage, diabetes and thrombosis.