The rising threat of antimicrobial resistance among pathogens highlights the critical need for novel antimicrobial agents. This study explores the potential of natural products by investigating hexane extracts from the marine sponge Haliclona fibulata (HF) for their antibacterial efficacy. The well diffusion method of HF extract showed significant antibacterial activity against P. aeruginosa, E. coli and S. aureus with inhibition zones of 20 ± 0.66 mm, 27 ± 0.58 mm, and 22 ± 0.33 mm, respectively. Scanning electron microscopy investigations confirmed the disruptive impact of the extracts on bacterial cell morphology. Further investigations using P. aeruginosa-infected zebrafish model revealed that HF treatment improved survival rates in a dose-dependent manner. Additionally, oxidative stress and apoptosis were significantly reduced in the 100 μg/mL (HF100) treatment group, alongside down-regulation of inflammatory markers such as il-1β, nf-kβ, tnf-α, and inos. Histological assessments showed reduced intestinal damage and fewer circulating macrophages in HF100-treated zebrafish larvae. Identifying potent bioactive compounds within the extracts suggests that HFcould be a valuable source of natural antimicrobials, warranting further exploration for developing treatments against pathogenic infections.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1002/cbdv.202402717 | DOI Listing |
Chem Biodivers
January 2025
SRM Institute of Science and Technology: SRM Institute of Science and Technology (Deemed to be University), Department of Chemical Engineering, Kattankulathur, 603203, Chengalpattu, INDIA.
The rising threat of antimicrobial resistance among pathogens highlights the critical need for novel antimicrobial agents. This study explores the potential of natural products by investigating hexane extracts from the marine sponge Haliclona fibulata (HF) for their antibacterial efficacy. The well diffusion method of HF extract showed significant antibacterial activity against P.
View Article and Find Full Text PDFMicrob Pathog
January 2021
Department of Microbiology, Alagappa University, Karaikudi, 630003, Tamil Nadu, India. Electronic address:
The alternative antimicrobial strategies that mitigate the threat of antibiotic resistance is the quorum-sensing inhibition (QSI) mechanism, which targets autoinducer dependent virulence gene expression in bacterial pathogens. N-acyl homoserine lactone (AHL) acts as a key regulator in the production of virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1 and violacein pigment production in Chromobacterium violaceum ATCC 12472. In the present study, the marine sponge Haliclona fibulata symbiont Brevibacterium casei strain Alu 1 showed potential QSI activity in a concentration-dependent manner (0.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!