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A dual expression plasmid with Microcin B17 compatible with both prokaryotic and mammalian systems.
MethodsX
June 2025
Department of Pharmacology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
Proteic plasmid addiction systems, such as the control of cell death (Ccd), have been used for efficient plasmid DNA recombination. The CcdB toxin, which has a relatively long sequence of 309 bp, has been the predominant choice for this purpose. However, the need for shorter peptide toxins has emerged.
View Article and Find Full Text PDFEffects of salt stress on plant and rhizosphere bacterial communities, interaction patterns, and functions.
Front Plant Sci
January 2025
Key Laboratory of Innovation of Forage Efficient Production Model, Ministry of Agriculture and Rural Affairs, Yinchuan, Ningxia Hui Autonomous Region, China.
Introduction: Salt stress significantly affects plant growth, and Na has gained attention for its potential to enhance plant adaptability to saline conditions. However, the interactions between Na, plants, and rhizosphere bacterial communities remain unclear, hindering a deeper understanding of how Na contributes to plant resilience under salt stress.
Methods: This study aimed to investigate the mechanisms through which Na promotes alfalfa's adaptation to salt stress by modifying rhizosphere bacterial communities.
Enhancing High-Level Food-Grade Expression of Glutamate Decarboxylase and Its Application in the Production of γ-Aminobutyric Acid.
J Microbiol Biotechnol
December 2024
School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, P.R. China.
Gamma-aminobutyric acid (GABA), a non-proteinogenic amino acid, exhibits diverse physiological functions and finds extensive applications in food, medicine, and various industries. Glutamate decarboxylase (GAD) can effectively convert L-glutamic acid (L-Glu) or monosodium glutamate (MSG) into GABA. However, the low food-grade expression of GAD has hindered large-scale GABA production.
View Article and Find Full Text PDFLong-Term Real-World Outcomes of Tenofovir Alafenamide in Chronic Hepatitis B: Detailed Analysis of Treatment-Naive and Experienced Patients.
Korean J Gastroenterol
January 2025
Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, Beijing International Cooperation Base for Science and Technology on NAFLD Diagnosis, Beijing, China.
Background/aims: This study assessed the long-term efficacy and safety of tenofovir alafenamide (TAF) in real-world settings.
Methods: Patients who were candidates for TAF treatment and were followed up at 12-week intervals over 192 weeks were enrolled in this study.
Results: One hundred and forty-four patients (50 treatment-naive and 94 treatment-experienced) were included in this study.
Maillard reaction inducing amino acids degradation can adjust the flavour characteristic of black tea.
Food Res Int
February 2025
Tea Research Institute, China Academy of Agricultural Sciences, Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Hangzhou 310008, China. Electronic address:
Drying is the step that is to be used to adjust and control the formation of flavour and quality in black tea processing. In the present work, the comprehensive two-dimensional gas chromatography with mass spectrometry (GC × GC-MS) and gas chromatography olfactometry with mass (GC-O-MS) were used to determine the dynamic change of the volatile compounds in black tea during drying at 90, 120, 150 °C for 1 h. Results showed that the ratio of esters and aldehydes largely declined when temperature was elevated from 90 °C to 150 °C, while the ratio of heterocycles was increased to 22.
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