Heavy metal contamination is a serious food safety issue. Herein, we report a rapid, multiplexed and naked-eye readable method for detecting heavy metal pollution in food samples using a cheap colorimetric paper, including milk. We leverage the urease catalysis reaction to amplify the presence of heavy metal ions, Hg and Pb, by exploiting their strong inhibitory effect on urease. Integrating with a paper-folding strategy, the visual assay realizes rapid detection of over 10 samples within 5 min. It allowed to detection of Hg as low as 0.1 nM and Pb as low as 2 μM, and the results can be read out either by naked eyes or smartphones. Results can be visually interpreted with the naked eye or a smartphone, making it practical for on-site testing and ensuring the safety of dairy products against metal contamination.
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| http://dx.doi.org/10.3168/jds.2024-25838 | DOI Listing |
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