In this work, we present a quantitative comparison of the cell division dynamics between populations of intact and regenerating root tips in the plant model system To achieve the required temporal resolution and to sustain it for the duration of the regeneration process, we adopted a live imaging system based on light-sheet fluorescence microscopy, previously developed in the laboratory. We offer a straightforward quantitative analysis of the temporal and spatial patterns of cell division events showing a statistically significant difference in the frequency of mitotic events and spatial separation of mitotic event clusters between intact and regenerating roots.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706681PMC
http://dx.doi.org/10.1017/qpb.2024.7DOI Listing

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