Multispectral transmission imaging has emerged as a promising technique for imaging breast tissue with high resolution. However, the method encounters challenges such as low grayscale, noisy transmission images with weak signals, primarily due to the strong absorption and scattering of light in breast tissue. A common approach to improve the signal-to-noise ratio (SNR) and overall image quality is frame accumulation. However, factors such as camera jitter and respiratory motion during image acquisition can cause frame misalignment, degrading the quality of the accumulated image. To address these issues, this study proposes a novel image registration method. A hybrid approach combining a genetic algorithm (GA) and a constriction factor-based particle swarm optimization (CPSO), referred to as GA-CPSO, is applied for image registration before frame accumulation. The efficiency of this hybrid method is enhanced by incorporating a squared constriction factor (SCF), which speeds up the registration process and improves convergence towards optimal solutions. The GA identifies potential solutions, which are then refined by CPSO to expedite convergence. This methodology was validated on the sequence of breast frames taken at 600 nm, 620 nm, 670 nm, and 760 nm wavelength of light and proved the enhancement of accuracy by various mathematical assessments. It demonstrated high accuracy (99.93%) and reduced registration time. As a result, the GA-CPSO approach significantly improves the effectiveness of frame accumulation and enhances overall image quality. This study explored the groundwork for precise multispectral transmission image segmentation and classification.
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http://dx.doi.org/10.3390/bioengineering11121281 | DOI Listing |
Front Cell Dev Biol
January 2025
Dipartimento di Scienze Biomediche e Cliniche, Università degli Studi di Milano, Milano, Italy.
Introduction: Endoplasmic reticulum aminopeptidases 1 (ERAP1) and 2 (ERAP2) modulate a plethora of physiological processes for the maintenance of homeostasis in different cellular subsets at both intra and extracellular level.
Materials And Methods: In this frame, the extracellular supplementation of recombinant human (rh) ERAP1 and ERAP2 (300 ng/ml) was used to mimic the effect of stressor-induced secretion of ERAPs on neutrophils isolated from 5 healthy subjects. In these cells following 3 h or 24 h rhERAP stimulation by Western Blot, RT-qPCR, Elisa, Confocal microscopy, transwell migration assay, Oxygraphy and Flow Cytometry we assessed: i) rhERAP internalization; ii) activation; iii) migration; iv) oxygen consumption rate; v) reactive oxygen species (ROS) accumulation; granule release; vi) phagocytosis; and vii) autophagy.
Plant Biotechnol J
January 2025
Institute of Plant Biotechnology and Cell Biology, Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
The production of complex multimeric secretory immunoglobulins (SIgA) in Nicotiana benthamiana leaves is challenging, with significant reductions in complete protein assembly and consequently yield, being the most important difficulties. Expanding the physical dimensions of the ER to mimic professional antibody-secreting cells can help to increase yields and promote protein folding and assembly. Here, we expanded the ER in N.
View Article and Find Full Text PDFInt J Nurs Stud
January 2025
NIHR Collaboration for Applied Research (Wessex), University of Southampton, Southampton, United Kingdom. Electronic address:
Ongoing challenges in the provision of care, driven by growing care complexity and nursing shortages, prompt us to reconsider the basis for efficient division of nursing labour. In organising nursing work, traditionally the focus has been on identifying nursing tasks that can be delegated to other less expensive and less highly educated staff, in order to make best use of scarce resources. We argue that nursing care activities are connected and intertwined.
View Article and Find Full Text PDFComput Biol Med
January 2025
State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, Tianjin, 300072, China. Electronic address:
Transmission imaging may become a possible advance for breast cancer screening with non-invasive, cost-effective, and radiation-free approaches for early detection. Frame accumulation can successfully eliminate the issue of low SNR, low grayscale and poor quality in transmission image. However, frame accumulation accuracy can be diminished because of inherent human body instability during image acquisition and the light absorption characteristics of breast tissue, resulting in distorted and misplaced image sequences.
View Article and Find Full Text PDFSensors (Basel)
December 2024
SOTI Aerospace, SOTI Inc., Mississauga, ON L5N 8L9, Canada.
Indoor navigation is becoming increasingly essential for multiple applications. It is complex and challenging due to dynamic scenes, limited space, and, more importantly, the unavailability of global navigation satellite system (GNSS) signals. Recently, new sensors have emerged, namely event cameras, which show great potential for indoor navigation due to their high dynamic range and low latency.
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