Background: , a green microalga, is a rich source of natural astaxanthin and a potent antioxidant with high commercial value. This study investigates the biological characteristics and potential of HB isolated from Hoa Binh, Vietnam, for growth and astaxanthin accumulation using a two-phase culture method.
Methods: HB was cultured in a C/RM medium at 25 °C, and morphological characteristics were examined. NMR spectroscopy was used to determine the structure of the astaxanthin, which was extracted using the Soxhlet method.
Results: After 22 days, the highest cell density (4.96 × 10 cells mL) was achieved under optimized light and ultraviolet conditions. Nutrient deprivation followed by bicarbonate supplementation resulted in a maximal astaxanthin accumulation of 48.8 mg g dry cell weight within two days. The extracted astaxanthin demonstrated potent antioxidant activity (IC: 3.74 mg mL) compared to ascorbic acid (IC: 18.53 µg mL) and exhibited strong acetylcholinesterase inhibition (IC: 297.99 µg mL). It also showed neuroprotective effects against HO and amyloid beta-induced neurotoxicity in C6 cells.
Conclusions: This study highlights HB as a promising source for large-scale astaxanthin production with potential applications in neuroprotective health products.
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| http://dx.doi.org/10.3390/bioengineering11121176 | DOI Listing |
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