Impact of Long-Term Cannabidiol (CBD) Treatment on Mouse Kidney Transcriptome.

Background: Cannabidiol, which is one of the main cannabinoids present in plants, has been shown to have therapeutic properties, including anti-inflammatory and antioxidant effects that may be useful for treatment of various kidney conditions.

Objectives: This article investigates the effect of long-term cannabidiol (CBD) treatment on changes in the renal transcriptome in a mouse model. The main hypothesis was that systematic CBD treatment would affect gene expression associated with those processes in the kidney.

Methods: The study was conducted on male C57BL/6J mice. Mice in the experimental groups received daily intraperitoneal injections of CBD at doses of 10 mg/kg or 20 mg/kg body weight (b.w.) for 28 days. After the experiment, kidney tissues were collected, RNA was isolated, and RNA-Seq sequencing was performed.

Results: The results show CBD's effects on changes in gene expression, including the regulation of genes related to circadian rhythm (e.g., , , , , and ), glucocorticoid receptor function (e.g., , , , , and ), lipid metabolism (e.g., , , , and ), and inflammatory response (e.g., and ).

Conclusions: The obtained results suggest that CBD may be beneficial for therapeutic purposes in treating kidney disease, and its effects should be further analyzed in clinical trials.