Background: Lipoxygenases (LOXs) are key enzymes in the unsaturated fatty acid oxidation reaction pathway and play an important regulatory role in the synthesis of fruit aroma volatiles.
Methods: gene family members were identified in the whole genome database of bitter gourd and analyzed bioinformatically. An RT-qPCR was used to analyze the expression differences in different tissues. Monoterpenes were determined by gas chromatography-mass spectrometry (GC-MS) technique.
Results: A total of 12 gene family members were identified in the genome. The expression of genes varied significantly among the tissues of roots, stems, leaves, flowers, fruits, seeds and tendrils. A total of 29 monoterpenes were detected in the fruits of five different fruit colors of bitter gourd, mainly containing six types of alcohols, aldehydes, terpenes, ketones, esters and alkynes, with the highest relative content of alcohols.
Conclusions: The present study provides a reference for further elucidation of the biological functions of the gene in the synthesis pathway of aroma volatiles in bitter gourd.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11675646 | PMC |
| http://dx.doi.org/10.3390/genes15121557 | DOI Listing |
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