N-glycome analysis of individual proteins and tissues is crucial for fundamental and applied biomedical research and medical diagnosis and plays an important role in the evaluation of the quality of biopharmaceutical and biotechnological products. The interest in this research area continues to grow annually, thereby increasing the demand for the high-throughput profiling of human blood plasma N-glycome. In response to this need, we have developed an optimized, simple, and rapid protocol for the N-glycome profiling of human plasma proteins. This protocol encompasses the entire analysis cycle, from plasma isolation to N-glycan spectrum quantification. While the proposed method may have lower efficiency compared to already published high-throughput methods, its adaptability makes it suitable for implementation in virtually any molecular biological laboratory.
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Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome.
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Institute of Cytology and Genetics SB RAS, Novosibirsk 630090, Russia.
N-glycome analysis of individual proteins and tissues is crucial for fundamental and applied biomedical research and medical diagnosis and plays an important role in the evaluation of the quality of biopharmaceutical and biotechnological products. The interest in this research area continues to grow annually, thereby increasing the demand for the high-throughput profiling of human blood plasma N-glycome. In response to this need, we have developed an optimized, simple, and rapid protocol for the N-glycome profiling of human plasma proteins.
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Department for Metabolism, Institute for the Application of Nuclear Energy, University of Belgrade, Belgrade, Serbia.
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School of Natural Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, NSW, Australia.
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Faculty of Medicine, Department of Physiology and Immunology, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, Osijek HR-31000, Croatia.
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CNR, Institute for Polymers, Composites and Biomaterials, IPCB, Catania, Italy.
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