Pyranose oxidase (POx) is an FAD-dependent oxidoreductase and belongs to the glucose-methanol-choline (GMC) superfamily of oxidoreductases. As recently reported, POxs and FAD-dependent -glycoside oxidases (CGOxs) share the same sequence space, and phylogenetic analysis of actinobacterial sequences belonging to this shared sequence space showed that it can be divided into four clades. Here, we report the biochemical characterization of a POx/CGOx from sp. 3H14 (POx), belonging to the hitherto unexplored clade II of actinobacterial POx/CGOx. Overall, POx demonstrates comparable features to POxs/CGOxs of clades III and IV, including the preference for glycosides over monosaccharides as electron donors. However, as POx efficiently oxidizes the -glycoside aspalathin as well as the -glycoside phlorizin, it shows activity with yet another set of glycoside structures compared to other POx/CGOx members.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3390/biom14121510 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice ( L.).
Plants (Basel)
December 2024
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
The plant hormone ethylene elicits crucial regulatory effects on plant growth, development, and stress resistance. As the enzyme that catalyzes the final step of ethylene biosynthesis, 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO) plays a key role in precisely controlling ethylene production. However, the functional characterization of the gene family in rice remains largely unexplored.
View Article and Find Full Text PDFCharacterization of a Pyranose Oxidase/-Glycoside Oxidase from sp. 3H14, Belonging to the Unexplored Clade II of Actinobacterial POx/CGOx.
Biomolecules
November 2024
Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU University, 1190 Vienna, Austria.
Pyranose oxidase (POx) is an FAD-dependent oxidoreductase and belongs to the glucose-methanol-choline (GMC) superfamily of oxidoreductases. As recently reported, POxs and FAD-dependent -glycoside oxidases (CGOxs) share the same sequence space, and phylogenetic analysis of actinobacterial sequences belonging to this shared sequence space showed that it can be divided into four clades. Here, we report the biochemical characterization of a POx/CGOx from sp.
View Article and Find Full Text PDFAnalysis of eIF4E-family members in Fungi contributes to their classification in eukaryotes.
J Biol Chem
December 2024
mRNA and Cancer Laboratory, Unit of Biomedical Research on Cancer, National Institute of Cancer (INCan), Mexico City 14080, Mexico.
The kingdom of fungi contains highly diverse species. However, fundamental processes sustaining life such as RNA metabolism are much less comparatively studied in Fungi than in other kingdoms. A key factor in the regulation of mRNA expression is the cap-binding protein eIF4E, which plays roles in mRNA nuclear export, storage and translation.
View Article and Find Full Text PDFUncovering novel functions of the enigmatic, abundant, and active in a salt marsh ecosystem.
mSystems
December 2024
Department of Earth, Ocean and Atmospheric Science, Florida State University, Tallahassee, Florida, USA.
, particularly uncultured representatives, are one of the most abundant microbial groups in coastal salt marshes, dominating the belowground rhizosphere, where over half of plant biomass production occurs. However, this class generally remains poorly understood, particularly in a salt marsh context. Here, novel metagenome-assembled genomes (MAGs) were generated from the salt marsh rhizosphere representing , , JAAYZQ01, B4-G1, JAFGEY01, UCB3, and orders.
View Article and Find Full Text PDFUnveiling the embryo structure in Bromeliaceae Juss. (Poales): morphological diversity, anatomy, and character evolution.
Ann Bot
December 2024
Laboratório de Anatomia Vegetal (LAVeg), Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, Porto Alegre, RS, Brazil.
Background And Aims: Recent studies have documented numerous morphoanatomical variations for the seed coat in Bromeliaceae. However, the structural diversity and character evolution of the embryo within this family remain largely unexplored. Given the embryo's significance in plant diversification, this research aims to investigate the morphology and key anatomical features of Bromeliaceae embryos, providing insights into character evolution, taxonomic applications, and reproductive biology.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!