Objective: This study aimed to assess the effect of implantation of fresh human amniotic membranes (HAM) on bone consolidation during distraction bone lengthening.
Methods: Ten New Zealand white rabbits were used in this study. For each rabbit, we performed a diaphyseal tibial osteotomy after installing a single-plane distraction external fixator. Rabbits were divided into 2 groups (n = 5): an osteotomy and rapid lengthening of 2.8 mm/days (negative control (NC) group) or an osteotomy with implantation of fresh HAM and rapid lengthening of 2.8 mm/days (HAM group). We carried out a rapid lengthening at a rate of 2 turns/day for 7 days and a single turn on the 8th day knowing that one turn corresponds to an elongation of 1.4 mm. The total elongation obtained was 21 mm corresponding to 20-25 % of the length of the rabbit's tibia. A radiological study was carried out on 4 and 8 weeks after the end of lengthening. Micro-computed tomography (μ-CT) analysis was carried out 8 weeks after the end of the lengthening and after the sacrifice of the rabbits associated with histological analyses.
Results: For the HAM group, radiographic analysis showed that all cases consolidated with a homogeneous callus visible at 1 month after lengthening, and complete corticalization was demonstrated at μ-CT analysis. Histological staining exhibited thick continuous cortices with intramedullary fibro adipose tissue with a medulla rich in hematopoietic cells.
Conclusion: The results show that HAM affects the stimulation of bone consolidation during bone lengthening by distraction.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jos.2024.11.011 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Drosophila cardiac myosin increases jump muscle stretch activation and shortening deactivation.
Biophys J
January 2025
Department of Biological Sciences & Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute Troy, NY 12180, USA; Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute Troy, NY 12180, USA. Electronic address:
Stretch activation (SA), a delayed increase in force production following rapid muscle lengthening, is critical to the function of vertebrate cardiac muscle and insect asynchronous indirect flight muscle (IFM). SA enables or increases power generation in muscle types used in a cyclical manner. Recently, myosin isoform expression has been implicated as a mechanism for varying the amplitude of SA in some muscle types.
View Article and Find Full Text PDFEffect of human amniotic membrane on the consolidation during bone lengthening by distraction: Experimental study in rabbits.
J Orthop Sci
January 2025
Cell Therapy and Experimental Surgery of Musculoskeletal System LR18SP11 Lab, Faculty of Medicine, Sfax, Tunisia; Department of Orthopedics and Traumatology, CHU Habib Bourguiba, Sfax, Tunisia.
Objective: This study aimed to assess the effect of implantation of fresh human amniotic membranes (HAM) on bone consolidation during distraction bone lengthening.
Methods: Ten New Zealand white rabbits were used in this study. For each rabbit, we performed a diaphyseal tibial osteotomy after installing a single-plane distraction external fixator.
In vivo assessment of shear modulus along the fibers of pennate muscle during passive lengthening and contraction using steered ultrasound push beams.
J Mech Behav Biomed Mater
December 2024
Nantes Université, Mouvement - Interactions - Performance, MIP, UR 4334, F-44000, Nantes, France; Institut Universitaire de France (IUF), Paris, France. Electronic address:
Ultrasound shear wave elastography (SWE) has emerged as a promising non-invasive method for muscle evaluation by assessing the propagation velocity of an induced shear wavefront. In skeletal muscles, the propagation of shear waves is complex, depending not only on the mechanical and acoustic properties of the tissue but also upon its geometry. This study aimed to comprehensively investigate the influence of muscle pennation angle on the shear wave propagation, which is directly related to the shear modulus.
View Article and Find Full Text PDFA mathematical framework for comparison of intermittent versus continuous adaptive chemotherapy dosing in cancer.
NPJ Syst Biol Appl
November 2024
Department of Mathematics, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Chemotherapy resistance in cancer remains a barrier to curative therapy in advanced disease. Dosing of chemotherapy is often chosen based on the maximum tolerated dosing principle; drugs that are more toxic to normal tissue are typically given in on-off cycles, whereas those with little toxicity are dosed daily. When intratumoral cell-cell competition between sensitive and resistant cells drives chemotherapy resistance development, it has been proposed that adaptive chemotherapy dosing regimens, whereby a drug is given intermittently at a fixed-dose or continuously at a variable dose based on tumor size, may lengthen progression-free survival over traditional dosing.
View Article and Find Full Text PDFTrypsin from digestive tract of harpiosquillid mantis shrimp: Molecular characteristics and the inhibition by chitooligosaccharide and its catechin conjugate.
J Food Sci
December 2024
International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Trypsin from the digestive tract of harpiosquillid mantis shrimp (HMS) was purified using ammonium sulfate precipitation and a soybean trypsin inhibitor-CNBr-activated Sepharose 4B affinity column. The purified trypsin (PTRP-HMS) had a purity of 30.4-fold, and a yield of 14.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!