AI Article Synopsis
- Low-temperature stress is a major challenge for apple production, and galactinol acts as a key cryoprotectant.
- Exogenous application of galactinol on apple saplings was shown to reduce damage from cold stress, and transgenic plants overexpressing the MdGolS5 gene exhibited increased cold tolerance.
- The study highlights the potential of galactinol and its biosynthetic pathway in enhancing cold resistance in apples, providing insights for future genetic improvements.
Article Abstract
Low-temperature stress is a limiting factor affecting the safe overwintering and stable production of apples. Galactinol, produced by galactinol synthase (GolS), is an important plant cryoprotectant. This study showed for the first time that exogenous spraying of apple saplings with 100 mg mL galactinol could effectively alleviate the damage from low-temperature stress. Further, we found that transgenic apple callus and tobacco overexpressing MdGolS5 showed strong cold tolerance. Specifically, the activities of antioxidant enzymes such as superoxide dismutase and GolS in transgenic tobacco overexpressing MdGolS5 increased under low-temperature treatment at -2 °C, and the contents of malondialdehyde, superoxide anion, and hydrogen peroxide were significantly lower than those of wild type tobacco. Moreover, large amounts of proline, galactinol, and raffinose were accumulated. In addition, the expression levels of cold-responsive genes MdCBF1, MdCBF2, MdCBF3, and MdCOR47 were significantly up-regulated in transgenic tobacco, further confirming the important role of MdGolS5 in regulating plant cold adaptation. In summary, this study not only revealed the direct effect of exogenous galactinol on the low-temperature protection of apple saplings for the first time, but also explored a new mechanism of raffinose family oligosaccharides anabolism in plant low-temperature adaptation through overexpression of MdGolS5. These results provide a theoretical basis for the genetic improvement of apple cold resistance.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.plaphy.2024.109416 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Galactinol synthase gene 5 (MdGolS5) enhances the cold resistance of apples by promoting raffinose family oligosaccharide accumulation.
Plant Physiol Biochem
December 2024
College of Horticulture, Shenyang Agricultural University, Key Lab of Fruit Quality Development and Regulation of Liaoning Province, Shenyang, 110866, China. Electronic address:
Article Synopsis
- Low-temperature stress is a major challenge for apple production, and galactinol acts as a key cryoprotectant.
- Exogenous application of galactinol on apple saplings was shown to reduce damage from cold stress, and transgenic plants overexpressing the MdGolS5 gene exhibited increased cold tolerance.
- The study highlights the potential of galactinol and its biosynthetic pathway in enhancing cold resistance in apples, providing insights for future genetic improvements.
Galactinol synthase 4 requires sulfur assimilation pathway to provide tolerance towards arsenic stress under limiting sulphur condition in Arabidopsis.
J Hazard Mater
December 2024
CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow 226001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India; CSIR-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), P.O. CIMAP, Near Kukrail Picnic Spot, Lucknow 226 015, India. Electronic address:
Heavy metalloid stress such as arsenic (As) toxicity and nutrient imbalance constitute a significant threat to plant productivity and development. Plants produce sulfur (S)-rich molecules like glutathione (GSH) to detoxify arsenic, but sulfur deficiency worsens its impact. Previous research identified Arabidopsis thaliana ecotypes Koz2-2 (tolerant) and Ri-0 (sensitive) under low-sulfur (LS) and As(III) stress.
View Article and Find Full Text PDFEfficacy and Fate of RNA Interference Molecules in the Green Pea Aphid, Acyrthosiphon pisum.
Arch Insect Biochem Physiol
December 2024
Biological Control of Insects Research Laboratory, Research Park, USDA Agricultural Research Service, Columbia, Missouri, USA.
RNA interference (RNAi) is a promising technology for controlling insect pests of agriculture. This technology is mediated through the application of double-stranded RNAs (dsRNAs), which are processed within the insect cells into small interfering RNAs (siRNAs). These molecules then target and reduce the expression of the insect-specific genes that can kill or reduce the performance of the pest.
View Article and Find Full Text PDFZmGolS1 underlies natural variation of raffinose content and salt tolerance in maize.
J Genet Genomics
December 2024
State Key Laboratory of Plant Environmental Resilience, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address:
Salt stress significantly inhibits crop growth and development, and mitigating this can enhance salt tolerance in various crops. Previous studies have shown that regulating saccharide biosynthesis is a key aspect of plant salt tolerance; however, the underlying molecular mechanisms remain largely unexplored. In this study, we demonstrate that overexpression of a salt-inducible galactinol synthase gene, ZmGolS1, alleviates salt-induced growth inhibition, likely by promoting raffinose synthesis.
View Article and Find Full Text PDFTranscriptome Analysis Reveals Genes Responsive to Three Low-Temperature Treatments in .
Plants (Basel)
November 2024
Laboratorio de Biotecnología Molecular de Plantas, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, A. C., San Luis Potosí, S.L.P. 78216, Mexico.
Cold stress impedes the growth and development of plants, restricts the geographical distribution of plant species, and impacts crop productivity. In this study, we analyzed the transcriptome to identify differentially expressed genes (DEGs) in 14-day-old plantlets exposed to temperatures of 0 °C, 4 °C, and 10 °C for 24 h, compared to the 22 °C control group. Among the top 50 cold-induced genes at each temperature, we identified 31 genes that were common across all three low temperatures, with nine genes common to 0-4 °C, eight genes to 4-10 °C, and two genes to 0-10 °C.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!