There is limited information on the occurrence of and ticks, as well as associated and species in Pakistan. Addressing this knowledge gap, the current study aimed at morphomolecular confirmation of these ticks and molecular assessment of associated Rickettsiales bacteria (, and spp.) in Balochistan, Pakistan. A total of 314 ticks were collected from 74 of 117 (63.2%) hosts, including 41 of 74 (55.4%) goats and 33 of 74 (44.5%) sheep. Subsequently, a subset of microscopically identified ticks was subjected to DNA extraction and PCR to amplify 16S rDNA and fragments. Additionally, , and fragments were targeted for spp. and 16S rDNA fragments for both and spp. The 16S rDNA and sequences of demonstrated 100% identity with those of the same species previously reported from Pakistan. The 16S rDNA and sequences of exhibited 99.52 and 100% identities, respectively, with corresponding species reported from China, Kazakhstan, and Turkey. The and fragments associated with . showed 100% identities with reported from Egypt, Italy, Kazakhstan, Kenya, Pakistan, and Senegal. The 16S rDNA sequences of sp. and sp. associated with both . and . exhibited 99.67 and 100% identities with unknown sp. and sp. reported from Morocco and Pakistan, respectively. In the 16S rDNA and phylogenetic trees of ticks, . and . from the current study clustered with their respective species. Similarly, in and phylogenetic trees of , of the present study clustered with the same species, whereas sp. and sp. of this study clustered with undetermined spp. and spp. in the 16S rDNA phylogenetic tree of Anaplasmataceae. Among the DNA samples from the screened ticks, a coinfection rate of , sp., and sp. (2 of 80, 2.5%) was observed in , whereas individual infection rates were noted as follows: (8 of 80, 10%), sp. (5 of 80, 6.3%), and sp. (5 of 80, 6.3%). This study marks the first record of molecular characterization of . and . as well as the detection of associated , sp., and sp. in Balochistan, Pakistan.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11701845 | PMC |
| http://dx.doi.org/10.3389/fvets.2024.1500930 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought Stress.
Microorganisms
December 2024
Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
Drought stress has a significant impact on agricultural productivity, affecting key crops such as soybeans, the second most widely cultivated crop in the United States. Endophytic and rhizospheric microbial diversity analyses were conducted with soybean plants cultivated during the 2023 growing season amid extreme weather conditions of prolonged high temperatures and drought in Louisiana. Specifically, surviving and non-surviving soybean plants were collected from two plots of a Louisiana soybean field severely damaged by extreme heat and drought conditions in 2023.
View Article and Find Full Text PDFAssessing Normandy Soil Microbial Diversity for Antibacterial Activities Using Traditional Culture and iChip Methods.
Microorganisms
November 2024
Université de Caen Normandie, CBSA UR 4312, UFR des Sciences, Campus 1, F-14000 Caen, France.
Uncultured microorganisms represent a promising and untapped source of antibacterial compounds, crucial in the fight against the significant threat of antimicrobial resistance (AMR). In this study, both traditional and isolation chip (iChip) cultivation techniques were employed to enhance the recovery of known and unknown microorganisms from soils located in Normandy, France. The isolates obtained were identified using 16S rDNA or ITS regions analysis and MALDI-TOF mass spectrometry and were screened for antibacterial activity.
View Article and Find Full Text PDFConservation and Dynamics of Maize Seed Endophytic Bacteria Across Progeny Transmission.
Microorganisms
November 2024
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Maize ( L.) is an important cereal crop species for food, feedstock and industrial material. Maize seeds host a suitable ecosystem for endophytic bacteria, facilitating seed germination and seedling growth.
View Article and Find Full Text PDFThe Impact of subsp. on Intestinal Microbial Community Composition and Diversity in Small-Tail Han Sheep.
Pathogens
December 2024
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, China.
Paratuberculosis (PTB), primarily caused by subsp. (MAP), is a chronic infection that affects ruminants and is difficult to prevent, diagnose, and treat. Investigating how MAP infections affect the gut microbiota in sheep can aid in the prevention and treatment of ovine PTB.
View Article and Find Full Text PDFDiversity and Characteristics of the Oral Microbiome Associated with Self-Reported Ancestral/Ethnic Groups.
Int J Mol Sci
December 2024
School of Dentistry, Meharry Medical College, Nashville, TN 37208, USA.
Periodontitis disproportionately affects genetic ancestral/ethnic groups. To characterize the oral microbiome from different genetic ancestral/ethnic groups, we collected 161 dental plaque samples from self-identified African Americans (AAs), Caucasian Americans (CAs), and Hispanic Americans (HAs) with clinical gingival health or biofilm-induced gingivitis on an intact periodontium. DNA was extracted from these samples, and then DNA libraries were prepared and sequenced using an Illumina NovaSeq high-throughput sequencer.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!