1Parkinson's disease (PD) involves the aggregation of the protein alpha-synuclein, a process promoted by interactions with intracellular membranes. To study this phenomenon in neurons for the first time, we developed a fluorescence lifetime imaging (FLIM) method using Förster resonance energy transfer and self-quenching reporters, analyzed with a custom-built FLIM microscope. This method offers insights into aggregate formation in PD and can be broadly applied to probe protein-membrane interactions in neurons.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702592 | PMC |
| http://dx.doi.org/10.1101/2024.12.19.629536 | DOI Listing |
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