AI Article Synopsis
- Five new pregnane C21-steroids were identified from the roots of Cynanchum bungei, including three previously unknown 5,6-epoxy steroids and two 8,14-seco-steroids.
- Compound 3 is notable for being the first 5a,6a-epoxy steroid discovered in Cynanchum plants, with their structures confirmed through various spectroscopic methods and theoretical calculations.
- All isolated compounds showed anti-inflammatory properties by inhibiting nitric oxide release in RAW264.7 cells, with compounds 3 and 4 demonstrating stronger activity than the commonly used anti-inflammatory drug indomethacin at a concentration of 50 μM.
Article Abstract
Five pregnane C21-steroids, including three 5,6-epoxy steroids (1-3) and two 8,14-seco-steroids (4 and 5), were isolated from the acid hydrolysate of Cynanchum bungei roots. Cynbungenins L-O (1-4) are previously undescribed compounds. Compound 3 with a 5a,6a-epoxy group represents the first example found in the Cynanchum plants. Their structures and absolute configurations were elucidated by a variety of spectroscopic analysis and theoretical ECD calculations. All compounds (1-5) were evaluated for their anti-inflammatory activity by inhibiting the lipopolysaccharide (LPS)-induced nitric oxide (NO) released in RAW264.7 cells. The results showed that they all possessed NO inhibitory activity at 50 μM, and compounds 3 and 4 exhibited stronger NO inhibitory activity than indomethacin.
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