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sp. nov., isolated from the shore soil of saline lake in Tibet of China. | LitMetric

sp. nov., isolated from the shore soil of saline lake in Tibet of China.

Int J Syst Evol Microbiol

China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.

Published: January 2025

AI Article Synopsis

  • Five new aerobic, Gram-negative bacterial strains were isolated from coastal soil at LungmuCo Lake in Tibet and are proposed as novel species based on genetic analyses.
  • Phylogenetic and genetic comparisons indicated these strains had high similarity to existing strains but were below the species delineation threshold.
  • Key characteristics included a unique genomic DNA G+C content, specific major polar lipids, and predominant fatty acids, leading to the proposal of the new species name for the strains, with C3-2-a3 identified as the type strain.

Article Abstract

Five aerobic, Gram-stain-negative bacterial strains, designated as C3-2-a3, B3-2-R+30, C3-2-a4, C3-2-M3 and C3-2-M8, were isolated from the coastal soil of LungmuCo Lake in the Tibet Autonomous Region, PR China. Phylogenetic analyses based on 16S rRNA genes and genomes indicated that these isolates belonged to the genus and showed a high similarity to LNNU 24178 (99.01%), RD2P54 (98.80%) and SJ-92 (97.67%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain C3-2-a3 and related reference strains LNNU 24178, RD2P54 and SJ-92 were 91.89, 83.11 and 83.86% and 46.90, 26.90 and 28.20%, respectively. All values were below the thresholds for delineating species, supporting their classification as novel species of the genus . The genomic DNA G+C content of strains C3-2-a3 was 68.39%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. The predominant respiratory quinone was ubiquinone-8 (Q-8), aligning with the characteristics of members of the genus . The major fatty acids (>10.0%) of strain C3-2-a3 were identified as iso-C, iso-C, iso-C and iso-C 9. Based on the results of phenotypic, physiological, chemotaxonomic and genotypic characterizations, we propose that the isolates represent a novel species of genus , for which the name p. nov is proposed. The type strain is C3-2-a3 (=CGMCC 1.14507=KCTC 8642).

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Source
http://dx.doi.org/10.1099/ijsem.0.006630DOI Listing

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