AI Article Synopsis
- Live imaging techniques provide real-time analysis of dynamic cellular processes in organisms, specifically studying the Drosophila ovary for various developmental phenomena like cell division and differentiation.
- A new extended ex vivo culture method has been developed for live imaging of female Drosophila germline stem cell (GSC) niches, allowing visualization of GSC asymmetric division and changes in spectrosome morphology.
- A detailed protocol is presented for this ex vivo culture, making it easier to study GSCs with various fluorescent tags commonly used in Drosophila research.
Article Abstract
Live imaging methods allow the analysis of dynamic cellular processes in detail and in real-time. The Drosophila ovary represents an excellent model to explore the dynamics of a myriad of developmental processes, such as cell division, stemness, differentiation, migration, apoptosis, autophagy, cellular adhesion, etc., over time. Recently, we have implemented an extended ex vivo culture and live imaging of the female Drosophila GSC niche. Using a Drosophila line harboring a GFP::Par-1 transgene as an example, this method allows the visualization of the GSCs' asymmetric division within their niche and the description of the changes in the spectrosome morphology along the cell cycle. Here, we present a detailed protocol for the ex vivo culture of Drosophila germaria, enabling prolonged visualization of the female GSC niche. Importantly, this protocol is broadly applicable to live imaging GSCs with multiple fluorescently tagged proteins of interest that are available in stock centers and/or in the Drosophila research community.
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| http://dx.doi.org/10.3791/67389 | DOI Listing |
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