AI Article Synopsis
- The study investigates the impact of areca nut chewing on oral squamous cell carcinoma (OSCC) by examining protein expression in affected tissues.
- Through advanced proteomic analysis, researchers identified 27 proteins that were differentially expressed in areca nut-related OSCC compared to non-areca nut-related cases and normal tissues.
- The findings highlight key proteins (LZTS1, MMP10, MYH6, MB, and TNNC1) as potential biomarkers for disease progression and therapy in areca nut-related OSCC.
Article Abstract
Objective: Areca nut chewing has been associated with a poor prognosis in oral squamous cell carcinoma (OSCC). This study seeks to identify differentially expressed proteins among areca nut-related OSCC,non-areca nut-related OSCC and adjacent normal epithelial tissues, with the aim of providing novel insights for the investigation of areca nut-related OSCC.
Methods: Using Tandem mass tag (TMT)-based proteomic analysis, a comparative proteomic profiling was conducted among areca nut-related OSCC, non-areca nut-related OSCC, and adjacent normal epithelial tissues (n=15). GO and KEGG enrichment analyses were then employed to identify significant proteins pertinent to the pathogenesis of OSCC for further study. Western Blot (WB) and Immunohistochemistry (IHC) techniques were used to preliminary validate the expression patterns of LZTS1, MMP10, MYH6, MB, and TNNC1 among various groups (n=30).
Results: 27 differentially expressed proteins were identified when comparing the areca nut-related OSCC group with both the non-areca nut-related OSCC and normal epithelial tissue groups. Among these, 15 proteins were upregulated, while 12 were downregulated. LZTS1 and MMP10 were included in the upregulated proteins, whereas MYH6, MB, and TNNC1 were downregulated. WB and IHC analyses corroborated the proteomic findings, revealing consistent expression trends for these 5 proteins across the studied groups.
Conclusion: LZTS1, MMP10, MYH6, MB and TNNC1 emerge as promising biomarkers for assessing disease progression, prognosis, and potential targeted therapies in areca nut-related OSCC.
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| http://dx.doi.org/10.1016/j.identj.2024.12.017 | DOI Listing |
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Article Synopsis
- The study investigates the impact of areca nut chewing on oral squamous cell carcinoma (OSCC) by examining protein expression in affected tissues.
- Through advanced proteomic analysis, researchers identified 27 proteins that were differentially expressed in areca nut-related OSCC compared to non-areca nut-related cases and normal tissues.
- The findings highlight key proteins (LZTS1, MMP10, MYH6, MB, and TNNC1) as potential biomarkers for disease progression and therapy in areca nut-related OSCC.
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