Protein-polyelectrolyte complexes (PPCs) exhibit opposite effects, both suppressing and promoting protein aggregation, depending on the type of components. For the application of PPCs as protein stabilization technology, these opposite effects must be controlled. In this study, we investigated the thermal aggregation of immunoglobulin G (IgG)-polyamino acid complexes to elucidate the relationship between the charge state of PPCs, evaluated by the zeta potential, and their opposite effects on the aggregation process, which were measured using a spectrophotometer. After heating the PPCs, with their zeta potential departing from neutral, the soluble fractions of IgG increased by about 60 % or more. Insoluble aggregation was likely suppressed because PPCs with highly charged surfaces tend to have difficulty associating with each other. Interestingly, after heating the PPCs, with their zeta potential approaching neutral, the monomer fractions of IgG increased to nearly 100 %. A lower charge on the PPC surface could lead to association between PPCs, resulting in a dense protein solution that stabilized the monomeric state of the protein. This simplicity of merely adjusting the surface charge of PPCs opens up a wide range of applications for controlling protein aggregation during PPC formation, a challenge that had previously been considered difficult to address.
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http://dx.doi.org/10.1016/j.ijbiomac.2025.139500 | DOI Listing |
Pharmaceutics
December 2024
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal.
The therapeutic management of melanoma, the most aggressive form of skin cancer, remains challenging. In the search for more effective therapeutic options, metal-based complexes are being investigated for their anticancer properties. Cisplatin was the first clinically approved platinum-based drug and, based on its success, other metals (e.
View Article and Find Full Text PDFPlants (Basel)
December 2024
Center for Applied Research on the Environment and Sustainability (CARES), School of Science and Engineering, The American University in Cairo, AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt.
Background: Mangroves are one of the key nature-based solutions that mitigate climate change impacts. Even though they are halophytic in nature, seedlings are vulnerable to high salinity for their establishment. This study investigated the effects of different salinities on seedling growth and mineral element composition of two dominant species ( and ).
View Article and Find Full Text PDFMicroorganisms
December 2024
Departamento de Ciencias y Geografía, Universidad de Playa Ancha, Avenida Leopoldo Carvallo 270, Playa Ancha, Valparaíso 2340000, Chile.
The Parque Nacional La Campana (PNLC) was recently recognized for its high soil surface microbial richness. Here, we explored the microbial community structure in soil profiles from contrasting facing slopes where sclerophyllous forest (SF) and xerophytic shrubland (XS) develop. Soil physicochemical conditions (dry density, pH, and organic matter C and N isotopic soil signatures) were determined at three depths (5, 10, and 15 cm depths).
View Article and Find Full Text PDFPathogens
December 2024
Department of Paediatrics, University College Dublin, CHI-Crumlin, D12 N512 Dublin, Ireland.
Metaproteomic analysis of microbiome post-translation modifications (PTMm) is challenging, and little is known about the effects of inflammation on the bacterial PTM landscape in IBD. Here, we adapted and optimised fluorescence in situ hybridisation-flow cytometry (FISH-FC) to study microbiome-wide tyrosine phosphorylation (p-Tyr) in children with and without inflammatory bowel disease (IBD). Microbial p-Tyr signal was significantly higher in children with IBD, compared to those without.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
State Key Laboratory of Mariculture Breeding, Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei University, Xiamen 361021, China.
Bidirectional promoters (BDPs) regulate the transcription of two adjacent, oppositely oriented genes, offering a compact structure with significant potential for multigene expression systems. Although BDPs are evolutionarily conserved, their regulatory roles and sequence characteristics vary across species, with limited studies in fish. Here, we systematically analyzed the distribution, sequence features, and expression patterns of BDPs in the medaka () genome.
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