Grass carp is an important commercial fish in China that is plagued by various diseases, especially the hemorrhagic disease induced by grass carp reovirus (GCRV). Autophagy, a highly conserved biological process among eukaryotes, is pivotal in maintaining cellular homeostasis and managing various stressors, including viral infections. Uncoordinated (Unc) 51-like kinase 2 (ULK2) is considered an initiator of the autophagic process. In this study, we successfully cloned and isolated the ULK2 gene from grass carp. We observed that its expression levels were markedly altered following exposure to GCRV or pathogen-associated molecular patterns (PAMPs). Overexpression of CiULK2 in grass carp ovary cells (GCO) promoted GCRV replication. Conversely, CiULK2 knockdown resulted in inhibited viral loads compared to the control group. Moreover, we also reveal that 2-aminoadipic acid (2-AAA), a representative autophagy related metabolite, can inhibit autophagy and viral replication. Notably, these roles of CiULK2 in autophagy and GCRV replication were reversed upon treatment with the 2-AAA. Collectively, our findings demonstrate the 2-AAA regulates CiULK2 to inhibit GCRV replication.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fsi.2024.110109 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cell-free hemoglobin released from hemolysis induces programmed cell death through iron overload and oxidative stress in grass carp (Ctenopharyngodon idella).
Fish Shellfish Immunol
January 2025
Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province 510222, China. Electronic address:
Intravascular hemolysis releases hemoglobin (Hb) from red blood cells under specific conditions, yet the effect of hemolysis in aquaculture systems remain poorly understood. In this study, a continuous hemolysis model for grass carp was established by injection of phenylhydrazine (PHZ) to investigate the mechanistic impacts of sustained hemolysis. PHZ-induced hemolysis altered liver color, and subsequent hematoxylin and eosin staining revealed substantial Hb accumulation in the head kidney, accompanied by inflammatory cell infiltration and vacuolization in liver tissue.
View Article and Find Full Text PDFEffects of ultrasound-assisted low-salt curing on water retention, tenderness and in vitro digestive characteristics of grass carp (Ctenopharyngodon Idellus).
Ultrason Sonochem
December 2024
Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, China; Hubei Engineering Research Center of Special Wild Vegetables Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi 435002, China. Electronic address:
The implementation of innovative techniques to achieve low-salt strategies in cured products is a critical issue faced by the food industry. This study aimed to investigate the impact of ultrasound treatment on the quality of the low-salt air-dried fish. The results showed that compared to traditional liquid curing, ultrasound-assisted curing significantly increased the NaCl transfer rate, improved tenderness, and improved water retention and in vitro digestibility (p < 0.
View Article and Find Full Text PDFThe 2-aminoadipic acid (2-AAA) regulates grass carp ULK2 to inhibit GCRV replication.
Fish Shellfish Immunol
January 2025
State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:
Grass carp is an important commercial fish in China that is plagued by various diseases, especially the hemorrhagic disease induced by grass carp reovirus (GCRV). Autophagy, a highly conserved biological process among eukaryotes, is pivotal in maintaining cellular homeostasis and managing various stressors, including viral infections. Uncoordinated (Unc) 51-like kinase 2 (ULK2) is considered an initiator of the autophagic process.
View Article and Find Full Text PDFEffects of (L.) fruit extract on the profile of hematology of ranchu goldfish ().
Open Vet J
November 2024
Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Article Synopsis
- The study investigates the effects of calabash fruit extract (BFE) on the growth and blood health of ranchu goldfish, showing that higher concentrations of BFE improve these aspects.
- Group P6, which received the highest BFE concentration, demonstrated the best growth performance and increased levels of specific immune cells (CD4+).
- The findings suggest that BFE could serve as an effective immunostimulant in fish diets, impacting both performance and hematological profiles positively.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!