AI Article Synopsis
- Bloodstream infections need quick bacterial identification for effective treatment, with traditional methods being slow.
- The study compared direct identification using MALDI-TOF MS with the post-culture method, finding that while direct identification had a lower success rate (64.8%), it provided results in under an hour.
- Despite its limitations, the direct method could significantly enhance diagnosis speed, making it a potentially valuable tool in conjunction with established methods.
Article Abstract
Introduction: Bloodstream infections are serious conditions requiring precise bacterial identification for effective treatment. Traditional culture-based methods, while reliable, are time-consuming. The direct identification method by MALDI-TOF MS promises rapid and accurate identification directly from positive blood cultures.
Aim: To evaluate and compare the direct MALDI-TOF MS identification method for positive blood culture samples with the post-culture MALDI-TOF MS method, which is currently recognized as the gold standard in bacteriological identification.
Methods: during the study period, 324 positive blood culture samples received at the Central Laboratory of Bacteriology, Serology, and Hygiene of the IBN SINA Hospital Center in Rabat were included in the study. Each sample was processed for microorganism identification by MALDI-TOF MS using both direct and post-culture methods.
Results: The direct identification method by MALDI-TOF MS showed a lower overall identification success rate (64.8%) compared to the post-culture method (100%). However, it allowed for bacterial identification in less than one hour without the need for a sub-culturing step, highlighting the technique's potential to enhance the diagnostic process.
Conclusion: The direct identification method by MALDI-TOF MS has the potential to improve the speed of bacterial identification in positive blood cultures compared to the current gold standard of identification after culture. Despite its limitations, the direct method offers an opportunity to improve diagnosis and patient management, especially when combined with the standard method.
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| http://dx.doi.org/10.62438/tunismed.v102i12.5221 | DOI Listing |
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