AI Article Synopsis
- Silymarin, a flavonoid from milk thistle, shows promising effects like antioxidant and anti-inflammatory properties, particularly in cancer research.
- In a study on SPC212 human mesothelioma cells, silymarin was found to have a dose-dependent cytotoxic effect, with a half-maximal inhibitory concentration (IC) of about 187.5 µM.
- The compound induced signs of apoptosis, such as cell shrinkage and nuclear changes, suggesting its potential as an anti-cancer agent that needs further investigation.
Article Abstract
Silymarin, a flavonoid complex isolated from Silybum marianum, possesses various biological properties, including antioxidant, anti-inflammatory, anti-glycation, and hepatoprotective effects. In the present study, we investigated the effects of silymarin on the SPC212 human mesothelioma cell line. MTT and neutral red assays were performed to examine the cytotoxic effects of silymarin. The apoptotic effect was investigated using AO/EB and DAPI staining, and morphological changes were observed using H&E and May-Grünwald staining. Additionally, immunocytochemistry was performed to detect Bax, Bcl2, and PCNA. Our results indicated that silymarin has a dose-dependent cytotoxic effect on SPC212 cells, with an IC value of approximately 187.5 µM. Silymarin induces apoptotic hallmarks such as apoptotic bodies, cell shrinkage, and nuclear condensation. In conclusion, silymarin demonstrated cytotoxic and apoptotic effects as well as morphological changes in SPC212 human mesothelioma cells. Further detailed studies are warranted to explore the potential of silymarin as an anti-cancer agent.
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| http://dx.doi.org/10.1007/s12013-024-01650-w | DOI Listing |
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Article Synopsis
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- The compound induced signs of apoptosis, such as cell shrinkage and nuclear changes, suggesting its potential as an anti-cancer agent that needs further investigation.
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