Ethnopharmacological Relevance: Yanghe Decoction(YHD) is a traditional Chinese medicine compound known for its efficacy in treating osteoarthritis (OA).
Aim Of The Study: We aimed to explore the underlying mechanisms of YHD in relation to OA.
Materials And Methods: UHPLC-MS technology was used to identify the material basis of YHD. In vivo, OA rat model was induced by the modified Hulth method and then treated with YHD at three different doses (0.625, 1.3 and 2.6g/kg/d). In vitro,YHD-Contained serum was prepared and administrated into rat chondrocytes, followed by simulation of Lipopolysaccharide(LPS). The protective mechanism was determined by observation of morphology, Flow cytometry and Protein level detection.
Results: In vivo, YHD reduced chondrocyte apoptosis and joint inflammation while promoting mitophagy. It also elevated the protein levels of p-AMPK, SIRT3, PINK1, Parkin, and LC3II/I. In vitro, YHD-Contained Serum reduced chondrocyte apoptosis, decreased mitochondrial ROS, enhanced mitochondrial membrane potential, and upregulated the protein expressions of p-AMPK, SIRT3, PINK1, Parkin, and LC3II/I.
Conclusion: Through this study, we demonstrated YHD protect chondrocytes against apoptosis, and its underlying mechanisms may involve the regulation of AMPK-SIRT3 positive feedback loop and activation of PINK1/Parkin mediated mitophagy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jep.2024.119294 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Yanghe decoction alleviates osteoarthritis by AMPK-SIRT3 positive feedback loop-mediated Mitochondrial autophagy.
J Ethnopharmacol
December 2024
Department of Orthopedics, The First Afliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, China; Engineering Research Center For Traditional Chinese Orthopedics Characteristic Technology and Equipment by Henan Province, Zhengzhou 450000, China. Electronic address:
Ethnopharmacological Relevance: Yanghe Decoction(YHD) is a traditional Chinese medicine compound known for its efficacy in treating osteoarthritis (OA).
Aim Of The Study: We aimed to explore the underlying mechanisms of YHD in relation to OA.
Materials And Methods: UHPLC-MS technology was used to identify the material basis of YHD.
Loss of protects against MASLD alone or with alcohol intake by preserving lipid homeostasis.
JHEP Rep
January 2025
Department of Immunology, Ophthalmology and ENT, Complutense University School of Medicine, Madrid, Spain.
Background & Aims: Expression of P21, encoded by the gene, has been associated with fibrosis progression in steatotic liver disease (SLD); however, the underlying mechanisms remain unknown. In the present study, we investigated the function of CDKN1A in SLD.
Methods: expression levels were evaluated in different patient cohorts with SLD, fibrosis, and advanced chronic liver disease (ACLD).
Wheat seedlings extract ameliorates sarcopenia in aged mice by regulating protein synthesis and degradation with anti-inflammatory and mitochondrial biogenesis effects.
Phytomedicine
July 2024
Center for Food and Nutritional Genomics Research, Kyungpook National University, 80, Daehak-ro, Buk-Ku, Daegu 41566, South Korea. Electronic address:
Background: Chronic inflammation, which becomes more prevalent during aging, contributes to sarcopenia by reducing muscle mass and strength.
Purpose: Wheat seedlings extract (WSE) is known for its various physiological activities, including anti-inflammation and antioxidant effects. However, its efficacy against sarcopenia is not well documented.
Mechanistic insights into AMPK-SIRT3 positive feedback loop-mediated chondrocyte mitochondrial quality control in osteoarthritis pathogenesis.
Pharmacol Res
April 2021
Department of Orthopaedics, Key Laboratory of Transplant Engineering and Immunology, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Osteoarthritis (OA) is a major cause of disability in the elderly population and represents a significant public health problem and socioeconomic burden worldwide. However, no disease-modifying therapeutics are currently available for OA due to an insufficient understanding of the pathogenesis of this disability. As a unique cell type in cartilage, chondrocytes are essential for cartilage homeostasis and play a critical role in OA pathogenesis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!