AI Article Synopsis
- CRISPR-Cas tools have been enhanced for tracking cell lineages during development, allowing for detailed analysis at single-cell resolution.
- scGESTALTv2 integrates CRISPR-Cas9 editing of a lineage barcode with single-cell RNA sequencing (scRNA-seq) to track cellular development.
- This method is applied in zebrafish brains, enabling the identification of cellular relationships among thousands of brain cells and various cell types.
Article Abstract
CRISPR-Cas tools have recently been adapted for cell lineage tracing during development. Combined with single-cell RNA sequencing, these methods enable scalable lineage tracing with single-cell resolution. Here, I describe, scGESTALTv2, which combines cumulative CRISPR-Cas9 editing of a lineage barcode array with transcriptional profiling via droplet-based single-cell RNA sequencing (scRNA-seq). The technique is applied in developing zebrafish brains to generate mutations in the barcode array during development. The recorded lineages along with cellular transcriptomes are then extracted via scRNA-seq to define cell relationships among thousands of profiled brain cells and dozens of cell types.
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| http://dx.doi.org/10.1007/978-1-0716-4310-5_15 | DOI Listing |
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