AI Article Synopsis
- The integrated portable and automatic digital detection system (IPADS) is a new tool designed for rapid and accurate diagnosis of infectious diseases through nucleic acid detection.
- It uses a hybrid magnetic system (HMS) to efficiently extract genetic material, simplifying traditional methods and allowing for quick sample processing with over 80% extraction efficiency.
- In tests for hepatitis B virus (HBV) DNA, IPADS showed strong alignment with standard PCR methods, proving its potential as a reliable option for point-of-care testing.
Article Abstract
The rapid, precise, and automated diagnosis of infectious diseases is crucial for effective disease management and control. Herein, the integrated portable and automatic digital detection system (IPADS), a novel diagnostic platform for nucleic acid detection is introduced. The device employs the hybrid magnetic system (HMS), which uses an electromagnet and a movable permanent magnet to modulate the magnetic field and control bead movement, increasing nucleic acid extraction efficiency to over 80%, while simplifying the traditional labor-intensive process and enabling quick, low-risk sample processing. Additionally, a disposable cartridge is designed for integrated HMS based preprocessing, with detection performed using digital RPA-Cas12a, enabling rapid, enclosed, and automation-friendly detection across a dynamic range spanning five orders of magnitude, with a sensitivity as low as 100 copies mL in serum samples. An automated platform further optimizes workflow. As a proof of concept, IPADS is applied to detect hepatitis B virus (HBV) DNA in 20 clinical serum samples, demonstrating high concordance with gold-standard quantitative PCR (qPCR) methods. These results validate the potential of IPADS as a reliable point-of-care testing solution.
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| http://dx.doi.org/10.1002/smtd.202401971 | DOI Listing |
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