AI Article Synopsis
- The study explores the potential of methylprednisolone-loaded nanoparticles (MP-NPs) in repairing spinal cord injuries by targeting a specific gene, SLC16A3, and altering the inflammatory response.
- Through analyzing gene expression data, researchers found that MP-NPs help promote a type of immune cell (M2 macrophages) that aids in recovery while protecting neurons and supporting the growth of neural stem cells.
- The results showed that MP-NPs improved motor function, reduced inflammation, and enhanced neural repair in rats with spinal cord injuries, suggesting they could be a new treatment approach.
Article Abstract
Objective: Spinal Cord Injury (SCI) leads to severe motor and sensory deficits, with limited treatment options. This study investigates how methylprednisolone-loaded nanoparticles (MP-NPs) modulate SCI repair by targeting Solute Carrier Family 16 Member 3 (SLC16A3) and reshaping the macrophage-inflammatory microenvironment.
Methods: Transcriptome data were analyzed to identify differentially expressed genes (DEGs) associated with SCI. Immune infiltration and WGCNA analyses identified genes linked to M2 macrophage polarization, pinpointing SLC16A3 as a key regulatory factor. MP-NPs were synthesized, characterized, and tested for their effects on macrophage polarization, neuronal protection, and SCI recovery in rats.
Results: We identified 612 DEGs related to inflammation and immune response in SCI. SLC16A3, upregulated in SCI, was downregulated by MP-NPs. In vitro, MP-NPs promoted M2 macrophage polarization, enhanced neuronal survival, and supported neural stem cell (NSC) differentiation. In vivo, MP-NPs significantly improved motor recovery, reduced inflammation, and facilitated neural repair in SCI rats.
Conclusion: MP-NPs downregulate SLC16A3 and modulate the macrophage-inflammatory environment, promoting neural repair and functional recovery in SCI, offering a promising therapeutic strategy.
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| http://dx.doi.org/10.14245/ns.2448814.407 | DOI Listing |
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