AI Article Synopsis
- Detecting circulating tumor cells (CTCs) is tough because they are present in low numbers and vary in characteristics, with traditional methods struggling for those with low EpCAM expression.
- This study introduces a new approach using silica-coated magnetic nanobeads with streptavidin for better CTC capture.
- The new method showed higher capture rates for specific cancer cell lines, especially those with low EpCAM expression, indicating its potential for improving CTC detection compared to existing commercial options.
Article Abstract
Detecting circulating tumor cells (CTCs) is challenging due to their low presence and heterogeneity. Traditional methods using EpCAM-based separation struggle with CTCs that have undergone epithelial-mesenchymal transition, as this results in lower EpCAM expression. This study presents the use of silica-coated magnetic nanobeads functionalized with streptavidin for CTC capture. Using the FETCH magnetic separation system, we validated the capture efficiency of our beads on tumor cells with varying EpCAM expression. Our beads showed superior capture rates for LNCaP (97%), PC3-9 (91%), PC3 (23%), A549 (22%), and T24 (8%) cells compared to commercial MojoSort™ beads. Despite slightly higher nonspecific binding than CellSearch, our beads demonstrated improved sensitivity for EpCAMlow cells, suggesting they have promise for enhanced CTC capture.
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| http://dx.doi.org/10.1002/1873-3468.15094 | DOI Listing |
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