An overview of recent progress in the molecular mechanisms and key biological macromolecules involved in limb regeneration of decapods.

Understanding the molecular mechanisms of limb regeneration in decapods can significantly enhance aquaculture production by improving survival and growth, as well as facilitating the development of lab-grown crustacean meat as a sustainable protein source. This review explores the molecular mechanisms of decapod limb regeneration, focusing on the key signaling pathways, genes, and proteins involved in this process. The initial stages of regeneration involve immune response and hemolymph coagulation, which are regulated via signaling pathways such as Toll, MAPK, IMD, and JAK/STAT. Subsequent stages, including blastema formation and limb growth, are regulated by signaling pathways such as Wnt, Hippo, Hedgehog, Ecdysteroid, TGF-β, Notch, Insulin-like, Fibroblast Growth Factor, Epidermal Growth Factor, and BMP. This review also discusses the interplay among environmental factors, nutrition, and hormonal signaling in regeneration and how these elements influence regenerative capability. Furthermore, this review highlights existing research gaps in decapod regeneration and suggests future research directions. This review aims to bridge existing gaps in decapod regeneration research and guide future studies toward potential breakthroughs in aquaculture practices.