To improve the efficiency of in-vitro-produced (IVP) porcine embryos, we focused on the events that usually occur during in-vivo embryonic transit from the oviduct to the uterus. Extracellular vesicles (EVs) are released by different mammalian cells and are imperative for intercellular communication and reflect the cell's physiological state. Based on these characteristics, EVs were isolated from oviductal and uterine fluid to imitate the in vivo environment and improve the efficiency of IVP embryos. Parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) embryos were divided into four groups based on treatment methods designed to mimic the in vivo migration pathways of porcine embryos. (Group 1) control group; (Group 2) a group treated with EVs from oviduct-derived fluid for 0-3 days (Ov-EVs), (Group 3) a group treated with EVs from uterus-derived fluid for 3-7 days (Ut-EVs); (Group 4) and a group treated with both (Ov, Ut-EVs). The EVs were characterized using various techniques, and their uptake into oocytes was confirmed using PKH67. The results demonstrated an increase in mitochondrial activity of PA embryos in Groups 2 and 4 at the 4-cell stage. Furthermore, compared with Group 1, the total number of cells in PA blastocysts was higher in the Group 2, 3 and 4, and the number of apoptotic cells was significantly lower. In SCNT experiments, the blastocyst development rate was increased in the EV-treated groups compared to the Group 1. Therefore, Ov-EVs and Ut-EVs can improve the embryonic development rate of IVP embryos, increase cell numbers and mitochondrial activity, and reduce apoptosis, thereby improving embryonic quality. Thus, integrating EV-based support into IVP embryos may advance swine reproductive technology and improve its practical applications.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.theriogenology.2024.12.020DOI Listing

Publication Analysis

Top Keywords

group group
16
ivp embryos
12
group treated
12
group
11
extracellular vesicles
8
oviductal uterine
8
uterine fluid
8
embryos
8
improve efficiency
8
porcine embryos
8

Similar Publications

Early developmental trajectories of the impaired hand in infants with unilateral cerebral palsy.

Dev Med Child Neurol

January 2025

Queensland Cerebral Palsy and Rehabilitation Research Centre, Child Health Research Centre, Faculty of Medicine, The University of Queensland, Brisbane, Australia.

Aim: To identify developmental trajectories of impaired hand function in infants aged 3 to 15 months with unilateral cerebral palsy (CP).

Method: Sixty-three infants (37 male; median gestational age 37 weeks [interquartile range 30-39.1 weeks]) recruited as part of a randomized trial with a confirmed diagnosis of unilateral CP were included.

View Article and Find Full Text PDF

Purposes: This meta-analysis aims to systematically analyze the efficacy of low-level red light (LRL) therapy for myopia control and prevention in children.

Methods: All the data were searched from the PubMed, EMBASE, and the Cochrane Library. The Cochrane Handbook was used to evaluate the quality of the included studies.

View Article and Find Full Text PDF

Diversity patterns and knowledge gaps of Atlantic Forest epiphyllous bryophytes: a highly neglected group.

Ann Bot

January 2025

Laboratório de Ecologia e Biogeografia de Plantas, Departamento de Biodiversidade, Setor Palotina, Universidade Federal do Paraná, Rua Pioneiro, 2153, Jardim Dallas, CEP 85950 000, Palotina, Paraná, Brazil.

Background: Epiphyllous bryophytes are a group of plants with complex adaptations to colonize the leaves of vascular plants and are considered one of the most specialized and sensitive groups to environmental changes. Despite their specificity and ecological importance, these plants represent a largely neglected group in relation to scientific research and ecological data. This lack of information directly affects our understanding of biodiversity patterns and compromises the conservation of this group in threatened ecosystems.

View Article and Find Full Text PDF

Study Objectives: Cancer-related fatigue is one of the most common symptoms in cancer survivors. Cognitive behavioural therapy for insomnia (CBT-I) can improve fatigue, but mechanisms are unclear. This secondary analysis of a randomized controlled trial evaluated whether CBT-I led to a significant improvement in fatigue, accounting for change in comorbid symptoms of insomnia, perceived cognitive impairment (PCI), anxiety, and depression.

View Article and Find Full Text PDF

Introduction: Prior research shows that in-person exposure to electronic nicotine delivery systems (ENDS) use increases desire for cigarettes and ENDS. However, less is known about the impact of cues delivered during remote interactions. This study extends previous in-person cue work by leveraging a remote confederate-delivered cue-delivery paradigm to evaluate the impact of dual nicotine vaping (vs.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!