High prevalence of antimicrobial resistance genes in multidrug-resistant-ESBLs-producing post-COVID-19 pandemic.

Background And Objectives: is a common pathogen associated with healthcare-related infections. It is particularly notable for its ability to develop resistance to multiple antibiotics, making treatment challenging. During the COVID-19 pandemic, increased antibiotic use to manage critically ill patients was contributed to the rise of multidrug-resistant . This study evaluated the antibiotic resistance patterns of multidrug-resistant, ESBL-producing in northern Iran after the COVID-19 pandemic.

Materials And Methods: This cross-sectional study was conducted between September 2022 and October 2023. Clinical samples were collected from patients with nosocomial infections at hospitals in Sari. This study included 114 multidrug-resistant ESBLs-producing isolates. Antimicrobial susceptibility was assessed using broth macro-dilution, and resistance genes were detected by multiplex PCR.

Results: Gentamicin, ampicillin-sulbactam, co-amoxiclav, and ceftazidime displayed the lowest activity against multi-drug-resistant . In contrast, piperacillin-tazobactam showed the highest activity. The prevalence of resistance genes was as follows: (99.12%), (74.56%), (88.60%), (64.04%), (92.98%), and (67.54%).

Conclusion: This study identified over 50% of antibiotic-resistance genes. Over half of multidrug-resistant isolates showed resistance to antibiotics except piperacillin-tazobactam, which is recommended for treating multi-drug-resistant infections.