Peony-shaped zinc oxide nanoflower synthesized via hydrothermal route exhibits promising anticancer and anti-amyloid activity.

Background: Cancer is the deadliest disease, and neurological disorders are also marked as slow progressive diseases, ultimately leading to death. Stopping two mouths with one morsel was the strategy that we used in this study.

Methods: We have synthesized peony-shaped zinc oxide nanoflowers (ZnO-NFs) and characterized them using various photophysical tools like UV-vis spectroscopy, zeta potential analysis, dynamic light scattering (DLS), FTIR, and scanning electron microscopy (SEM), and utilized these nanoflowers to monitor their anticancer and anti-amyloid activity. In vitro biocompatibility was assessed using fibroblasts and undifferentiated rat phaeochromocytoma cells, and in vivo, biocompatibility was estimated using haemolysis assay and zebrafish embryo development.

Results: The results demonstrated high biocompatibility of the as-synthesized ZnO-NFs up to a dose of 200 µg/ml. In vitro anticancer activity was evaluated using adherent (A375) and non-adherent (Dalton's Lymphoma Ascites, DLA) cancer cell lines. The results indicated that the ZnO-NFs significantly killed the cancer cells in a dose-dependent way, showing an extraordinary effect on DLA cells. The anti-amyloid activity in vitro was explored using a spectrum of assays that were hallmarks in anti-amyloid studies like ThT fluorescence assay, DLS, turbidity assay, atomic force microscopy (AFM), and SEM analysis. Excellent anti-amyloid activity was observed in vitro at 50 µg/ml of ZnO-NFs.

Conclusion: We can conclude from the above results that the as-synthesized ZnO-NFs have a dual role as an anticancer as well as an anti-amyloid agent. In the future, animal models can be used to study the efficacy of the ZnO-NFs in cancer inhibition and amyloid degradation.