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Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria.
J Vis Exp
December 2024
Institute for Synthetic Microbiology, Heinrich Heine University Düsseldorf; Department of Biochemistry and Molecular Biology, Michigan State University.
With the increasing demand for sustainable biotechnologies, mixed consortia containing a phototrophic microbe and heterotrophic partner species are being explored as a method for solar-driven bioproduction. One approach involves the use of CO2-fixing cyanobacteria that secrete organic carbon to support the metabolism of a co-cultivated heterotroph, which in turn transforms the carbon into higher-value goods or services. In this protocol, a technical description to assist the experimentalist in the establishment of a co-culture combining a sucrose-secreting cyanobacterial strain with a fungal partner(s), as represented by model yeast species, is provided.
View Article and Find Full Text PDFMaking the Case for an International Childhood Cancer Data Partnership.
J Natl Cancer Inst
January 2025
Surveillance Research Program, Division of Cancer Control and Population Sciences, National Cancer Institute, Rockville, MD, USA.
Childhood cancers are a heterogeneous group of rare diseases, accounting for less than 2% of all cancers diagnosed worldwide. Most countries, therefore, do not have enough cases to provide robust information on epidemiology, treatment, and late effects, especially for rarer types of cancer. Thus, only through a concerted effort to share data internationally will we be able to answer research questions that could not otherwise be answered.
View Article and Find Full Text PDFTitle: Can ChatGPT 4.0 Reliably Answer Patient Frequently Asked Questions About Boxer's Fractures?
Hand Surg Rehabil
January 2025
Department of Orthopaedic Surgery, Icahn School of Medicine at Mount Sinai, New York City, NY, United States.
Background: Patients are increasingly turning to the internet, and recently artificial intelligence engines (e.g., ChatGPT), for answers to common medical questions.
View Article and Find Full Text PDFSARS-CoV-2 Vaccination Can Only Be Held Responsible for Thrombotic Thrombocytopenic Purpura After All Differentials Have Been Ruled Out.
HCA Healthc J Med
December 2024
Neurology and Neurophysiology Center, Vienna, Austria.
Description This letter to the editor addresses limitations to holding COVID vaccines responsible for the diagnosis of thrombotic thrombocytopenic purpura and discusses the potential differential diagnoses that must be considered.
View Article and Find Full Text PDFA Commentary on "Full-Endoscopic Resection of a Lumbar Intradural Tumor (Schwannoma): Video Case Report and Description of the Surgical Technique".
Neurospine
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Department of Neurosurgery, Kyung Hee University Hospital at Gangdong, Kyung Hee University College of Medicine, Seoul, Korea.
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