Nuclear Receptor Subfamily 4 Group A Member 3: A Potential Marker of Endometriosis.

Background: Nuclear receptor subfamily 4 group A member 3 () is lowly expressed in ectopic endometrium and can be degraded by ubiquitination in vascular endothelial cells. Murine double minute 2 () is predicted to be the ubiquitin ligase of . Hence, we investigated the effects of and on endometriosis and clarified corresponding regulatory mechanisms.

Methods: The ubiquitin ligase of was predicted using bioinformatics and validated by immunoprecipitation. The effects of and on the migration and proliferation of human endometrial stromal cells (hESCs) were examined by Transwell assay and 5-ethynyl-2'-deoxyuridine (EdU) staining. and expressions were detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. An endometriosis model was constructed in Sprague-Dawley rats, followed by body weight analysis, ultrasonic imaging of ectopic cysts, and Western blot.

Results: Overexpression of inhibited, but siNR4A3 boosted hESC migration and proliferation. promoted ubiquitination and degradation. overexpression enhanced hESC migration and proliferation and partially reversed the inhibitory effect of overexpression. Overexpression of reduced ectopic cysts in endometriotic rats, which was offset by overexpression.

Conclusion: , which is promoted to ubiquitination and degradation by , inhibits the proliferation and migration of hESCs , and reduces the growth of ectopic endometrial cysts , thereby inhibiting the progression of endometriosis.