[Simultaneous Removal of Antibiotic-resistant Bacteria, Genes, and Inhibition of Horizontal Transfer using Vis-rGO-CNCF-enhanced Peroxymonosulfate Activation Process].

As emerging contaminants, antibiotic-resistant bacteria （ARBs） and antibiotic-resistant genes （ARGs） pose a serious threat to human health and ecological security. Here, a reduced graphene oxide and g-CN co-doped copper ferrite （rGO-CNCF） were synthesized. The composite material was characterized using XRD, FTIR, XPS, SEM-EDS, TEM, and DRS analysis methods, and a visible-light-assisted rGO-CNCF-activated PMS system was constructed for the removal of ARB and ARGs in water. The results showed that the complete inactivation of 8.01 log SA-ARB could be achieved within 30 min when the catalyst dosage was 0.2 g·L, The PMS dosage was 0.3 g·L, and the initial pH value of the solution was 7.0. The Vis-rGO-CNCF/PMS system was able to effectively reduce the horizontal transfer of SA-ARGs, and this system had a good destructive ability for intracellular and extracellular SA-ARGs. The destruction ability of the advanced oxidation process for the two pollutants together, SMT and SA-ARB, was maintained at a high level. This system could destroy the cell membrane structure of resistant bacteria, causing cell fragmentation, and quenching experiments showed that singlet oxygen （O） played a major role in the system. This study can provide a promising method for controlling ARB and ARG pollution in water and controlling the horizontal transfer of ARGs.