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Monitoring HO levels in live cells is essential due to its superior stability and possible severity inside the cell. The quest for a superior platform capable of detecting cellular-level hydrogen peroxide (HO) concentrations without necessitating the use of high-cost enzymes is of utmost importance. Here, the quantification of intracellular HO concentrations has been performed using silver metal polymer-based nonenzymatic electrochemical detection. Two forms of silver metal-organic polymers are synthesized to explore its suitability as catalytic nanozyme activity for cell-level HO detection with good accuracy. An Ag-O coordination interaction-based silver metal-organic framework, AgMOF-5 was synthesized and then transformed into a compact silver nanosphere (Ag nanosphere), rich in Ag-N coordination bond, to find the potential of individual coordination arrangement of each material in favour of the electrical conductivity, catalytic property, and sensing ability. AgMOF-5 modified Glassy Carbon Electrode (AgMOF/GCE) was found to be highly conductive while also demonstrating exceeding catalytic potential towards both commercial HO and cell-secreted HO with a LOD of 0.7 nM and sensitivity of 1300 μA mMcm. AgMOF/GCE demonstrated superior sensor characteristics like superconductivity, high stability, high reproducibility, and good selectivity upon investigation. Among many cellular byproducts of metabolism, HO and other ROS species indicate the oxidative stress and are a key differentiator between healthy and diseased conditions. The modified GCE was used for live monitoring of HO levels in L929, HeLa, T98G, and LN18 cell lines. The detection of higher HO levels in glial cells established its predisposition towards increased oxidative stress. The electrochemical results of AgMOF/GCE were validated against a Phenol Red, HRPO-based live cell compatible Colourimetric detection for applicability and acceptance of AgMOF/GCE as HO detection platform.
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| http://dx.doi.org/10.1016/j.bios.2024.117056 | DOI Listing |
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