AI Article Synopsis
Article Abstract
Low-cost production of cellulases is a key factor in advancing the commercialization of lignocellulosic biorefinery. Thus far, Trichoderma reesei is the leading cellulase producer for biorefinery applications. Over 70 years of research, considerable advancements have been made in comprehending the mechanisms underlying cellulases biosynthesis and secretion in T. reesei, as well as enzymatic cellulose hydrolysis. However, many unknowns still hinder the rational design of strains for robust cellulase production, with an optimized ratio of cellulolytic enzymes to reduce the required dosage for cellulose hydrolysis. Moreover, large-scale cellulase production relies on submerged fermentation, which suffers from several mass transfer limitations. As the mycelia grow, the fermentation broth rapidly develops non-Newtonian properties, necessitating energy-intensive mixing and aeration to facilitate oxygen transfer essential for strain growth. Herein, this paper critically reviews updated progress in these regards, highlights challenges, and outlines potential solutions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biortech.2024.132015 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mechanism-based Modelling for Fitting the Double-exponential Progress Curves of Cellulase Reaction.
J Appl Glycosci (1999)
November 2024
1 Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo.
Enzymatic hydrolysis of cellulosic biomass is a complex process involving many factors, including multiple enzymes, heterogeneous substrates, and multi-step enzyme reactions. Cellulase researchers have conventionally used a double-exponential equation to fit the experimental time course of product formation, but no theoretical basis for this has been established. Here we present a mechanism-based equation that fits well the progress curves of cellulase reaction, incorporating the concepts of non-productive and productive binding on the cellulose surface and processivity.
View Article and Find Full Text PDFCutting-edge advances in strain and process engineering for boosting cellulase production in Trichoderma reesei.
Bioresour Technol
December 2024
Key Laboratory of Shandong Microbial Engineering, School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250353, People's Republic of China; Department of Chemical and Petroleum Engineering, University of Calgary, 2500 University Drive, NW, Calgary, Alberta, Canada. Electronic address:
Low-cost production of cellulases is a key factor in advancing the commercialization of lignocellulosic biorefinery. Thus far, Trichoderma reesei is the leading cellulase producer for biorefinery applications. Over 70 years of research, considerable advancements have been made in comprehending the mechanisms underlying cellulases biosynthesis and secretion in T.
View Article and Find Full Text PDFA Comparison of Digestive Strategies for With Different Diet Compositions: Digestive Enzyme Activities, Gut Microbiota, and Metabolites.
Ecol Evol
December 2024
Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Biology, College of Life Sciences Xinjiang Agricultural University Urumqi China.
Animal gut microbiota play important roles in host immunity, nutrient metabolism, and energy acquisition. The gut microbiota and its metabolic products interact with the host in many different ways, influencing gut homoeostasis and health. is an endemic species which displays special frugivorous behavior, and it has been observed consuming grapes.
View Article and Find Full Text PDFAnalysis of the effects of HJ-16 inoculation on tobacco leaves based on multi-omics methods.
Front Bioeng Biotechnol
December 2024
State Key Laboratory of Crop Stress Adaptation and Improvement, College of Agriculture, Henan University, Kaifeng, China.
In this study, a strain isolated from the surface of flue-cured tobacco leaves, identified as HJ-16, was applied in the solid-state fermentation of tobacco leaves. This strain, known for producing thermally stable enzymes, including amylase, cellulase, and protease, significantly improved the sensory qualities of tobacco, enhancing aromatic intensity, density, and softness, while reducing irritation. Whole-genome sequencing and functional annotation revealed that HJ-16 possesses a single circular chromosome containing genes associated with enzyme production and metabolic activities, particularly in carbohydrate metabolism and amino acid metabolism.
View Article and Find Full Text PDFEnzymatic treatment shapes in vitro digestion pattern of phenolic compounds in mulberry juice.
Food Chem
December 2024
Department of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address:
The health benefits of mulberry fruit are closely associated with its phenolic compounds. However, the effects of enzymatic treatments on the digestion patterns of these compounds in mulberry juice remain largely unknown. This study investigated the impact of pectinase (PE), pectin lyase (PL), and cellulase (CE) on the release of phenolic compounds in whole mulberry juice.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!