Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Objectives: In the present study, we investigate the effect of FeO nanoparticles conjugated with ursolic acid (FeONPs@UA) on inhibiting the growth, biofilm-forming ability and efflux pump activity in clinical isolates of Pseudomonas aeruginosa with multiple drug resistance.
Methods: Iron oxide NPs conjugated with ursolic acid (FeONPs@UA) were synthesized. Physicochemical features of the NPs were studied by FT-IR, XRD, EDAX, and TEM. The antibacterial and antibiofilm effects of FeONPs@UA against P. aeruginosa isolates were determined by broth microdilution and microtiter plate methods, respectively. The efflux pump inhibitory effect of FeONPs@UA was determined using Cartwheel method and through determining the expression level of efflux pump genes, including mexA and oprD in selected P. aeruginosa isolates treated with sub-MIC concentration of FeONPs@UA by real-time PCR.
Results: In investigating the antimicrobial effect of FeONPs@UA, the MIC of these nanoparticles varied between 0.19 and 0.78 mg/mL and in the study of the anti-biofilm effect of FeONPs@UA, it caused a 68-75 % decrease in biofilm formation compared to the control. Moreover, in the Cartwheel test, the anti-efflux effect of these nanoparticles was confirmed at 1/4-MIC concentrations, and the expression of mexA and oprD genes was reduced in bacteria treated with FeONPs@UA compared to the control.
Conclusion: According to the results, the use of FeONPs@UA can provide a basis for the development of new treatments against drug-resistant bacteria in P. aeruginosa. This substance can improve the concentration of antibiotics in bacterial cells and increase their effectiveness by inhibiting the efflux in P. aeruginosa isolates.
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Source |
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http://dx.doi.org/10.1016/j.micpath.2024.107241 | DOI Listing |
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