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Immunoaffinity Depletion of High-Abundance Proteins from Serum/Plasma for Proteomic Analysis. | LitMetric
Mass spectrometry-based proteomics is widely applied to human blood serum or plasma in the search of biomarkers for various diseases. However, the enormous complexity and dynamic range of protein concentrations in these samples render a significant analytical challenge, particularly for detecting low-abundance candidate biomarkers. As a result, strategies for enriching low-abundance proteins and improving their identification in serum or plasma proteomics are commonly used. Here, we describe an immunodepletion technique that is routinely used in our lab for removing high-abundance proteins from human serum/plasma.
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